SARS-CoV-2 Spike Mutants

Recombinant antigens and lentivirus for emerging SARS-CoV-2 variants

During the course of COVID-19 pandemic, several new SARS-CoV-2 variants have emerged, each with differing mutations that can have impacts on transmissibility, COVID-19 disease severity, and effectiveness of vaccines. AMSBIO now offers a series of recombinant antigens and pseudotyped lentivirus with key Spike mutations in order to evaluate the efficacy of antibodies and vaccines. These products form part of our larger COVID-19 Tools for Research portfolio, including other high quality recombinant proteins, Spike pseudotyped lentivirus, and reagents for vaccine development and evaluation.

Spike Mutant Recombinant Proteins

B.1.1.7 and B.1.351 Variants

The B.1.1.7 variant strain (also known as 20B/501Y.V1, VOC 202012/01) of SARS-CoV-2 was first discovered in the United Kingdom in late 2020 with an unusually large number of mutations, including a 69/70 deletion and P681H. Following this, the variant has been detected in multiple countries around the world, including the United States, and is associated with increased transmissibility.

In South Africa, another variant of SARS-CoV-2 (known as 20C/501Y.V2 or B.1.351 lineage) emerged independently of the B.1.1.7 variant. This variant shares some mutations with the B.1.1.7 lineage, but does not contain the deletion at 69/70.

We now provide both B.1.1.7 and B.1.351 lineage SARS-CoV-2 Spike recombinant antigens for COVID-19 research.

NamePacksize Order
SARS-CoV-2 (COVID-19) S protein RBD (E484K), His Tag (MALS verified)1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (E484K), His Tag (MALS verified)100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified)1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified)100 ug View
SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag100 ug View
SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, N501Y, D614G), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, N501Y, D614G), His Tag100 ug View

Upcoming Proteins

These proteins listed below are currently in the pipeline. Contact us if you are interested in any of these antigens and we can provide further information on the product and its availability.

Protein Cat. # Tag Host Variant Mutations Register Interest
S protein AMS.SPN-C52Hc His Tag HEK293 B.1.351 L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V Contact us if you are interested
AMS.SPN-C52H6 B.1.1.7 HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H Contact us if you are interested
S1 protein AMS.S1N-C52Hd B.1.1.7 HV69-70del Contact us if you are interested
AMS.S1N-C52Hc Y144del Contact us if you are interested
AMS.S1N-C52Hg B.1.1.7/B.1.351 N501Y Contact us if you are interested
AMS.S1N-C52Hb B.1.1.7 P681H Contact us if you are interested
S1 protein NTD AMS.S1D-C52H9 B.1.351 L18F, D80A, D215G, R246I Contact us if you are interested
S protein RBD AMS.SPD-C52Hn B.1.1.7/B.1.351 N501Y Contact us if you are interested
Nucleocapsid protein AMS.NUN-C52H8 B.1.1.7 D3L, S235F Contact us if you are interested

Other Spike Mutants

Our B.1.1.7 and B.1.351 Spike mutant recombinant antigens are also complemented by Spike antigens with other key mutations, including N234Q, V367F, and N354D.

NamePacksize Order
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitagâ„¢, Super stable trimer (MALS verified)200 ug View
Biotinylated SARS-CoV-2 S protein (D614G), His,Avitagâ„¢, Super stable trimer (MALS verified)25 ug View
SARS-CoV-2 (COVID-19) S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (under development)50 ug View
SARS-CoV-2 (COVID-19) S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (under development)500 ug View
SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)50 ug View
SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)500 ug View

NamePacksize Order
Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitagâ„¢200 ug View
Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitagâ„¢25 ug View
SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag100 ug View
SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag100 ug View
SARS-CoV-2 (COVID-19) S1 protein (N501Y), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein (N501Y), His Tag100 ug View

NamePacksize Order
SARS-CoV-2 (COVID-19) S1 protein NTD (N234Q), His Tag1 mg View
SARS-CoV-2 (COVID-19) S1 protein NTD (N234Q), His Tag100 ug View

NameDatasheetPacksize Order
SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (G476S), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (G476S), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified)-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified)-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (L452R), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (L452R), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (N439K), His Tag (MALS verified)-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (N439K), His Tag (MALS verified)-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His Tag (MALS verified)-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His Tag (MALS verified)-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (S477N), His Tag (MALS verified)-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (S477N), His Tag (MALS verified)-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (V483A), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (V483A), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag-100 ug View
SARS-CoV-2 (COVID-19) S protein RBD (Y453F), His Tag (MALS verified)-1 mg View
SARS-CoV-2 (COVID-19) S protein RBD (Y453F), His Tag (MALS verified)-100 ug View

Spike Mutant Pseudotyped Lentivirus

SARS-CoV-2 Spike D614G Pseudotyped Lentivirus

We provide ready-to-use SARS-CoV-2 Spike Pseudotyped Lentivirus with D614G mutation. This mutation emerged in a variant of SARS-CoV-2 in late January or early February 2020 and was discovered to dramatically increase infectivity of the virus. SARS-CoV-2 viruses carrying this mutation have spread rapidly around many parts of the world due to this transmission advantage. This lentivirus is produced with SARS-CoV-2 Spike with D614G mutation as the envelope glycoproteins instead of the commonly used VSV-G, and can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility. For further information on our other SARS-CoV-2 Spike Pseudotyped Lentiviruses, refer to this page.

NameDatasheetPacksize Order
Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (Luc Reporter)100 ul View
Spike (SARS-CoV-2, D614G) Pseudotyped Lentivirus (Luc Reporter)500 ul x 2 View

Other Spike Mutant Pseudotyped Lentivirus

Spike pseudotyped lentivirus with other key mutations, including N439K, P618H, K417N, E484K and N501Y, can be produced through our Custom Lentivirus Service. These mutations have received a lot of attention due to their ability to either enable the virus to evade antibody-mediated immunity or increase virus infectivity. If you require other Spike mutant pseudotyped lentivirus, you can request these using our Custom Lentivirus Service. Find out more here.