Recombinant Monoclonal Antibody Service

Rapid and efficient monoclonal antibody development

Our high quality recombinant monoclonal antibody development services offer an superior alternative to hybridoma fusion platforms, as we directly isolate IgG genes from B cells of immunized animals. This enables us to produce the antibodies you need in as little as 4-5 months. With both mouse and rabbit monoclonal antibody development available, we can tailor our service to directly suit your research needs. We also provide thousands of high quality off-the-shelf monoclonal and polyclonal antibodies, browse our collection here.

Benefits

    • Short development process (4-5 months)
    • High project success rate (80-90%)
    • High cloning efficiency – thousands of clones from one immunized animal
    • Direct acquisition of antibody IgG gene sequence, easy to maintain and transmit information
    • High quality consistency between batches, and possible to automate the process

Applications

    • Therapeutic antibody drug development
    • Immunohistochemistry
    • Companion diagnostics
    • Immunoblotting
    • Flow cytometry

Our Services

Comparison between rabbit and mouse monoclonal antibodies

FeatureRabbit Monoclonal AntibodiesMouse Monoclonal Antibodies
Affinity+++++++
New IHC clones++++++++
Old and common IHC clones+++++++
Maturity for antibody drug development+++++++
Common IVD clones++++++

Rabbit Monoclonal Antibody Development

Rabbit monoclonal antibodies are increasing in popularity for use in immunological assay development, particularly in immunohistochemistry (IHC) applications. Currently, only 5 FDA approved companion diagnostic IHC antibodies are rabbit monoclonal antibodies. However, this new proprietary technology platform offers opportunity for a great technology breakthrough in this area, with shortened development time and acquisition of IgG gene sequence without the loss of hybridoma cells.

Service Details and Deliverables

StepService DetailsDeliverablesEstimated Production Cycle
Immunogen preparationPlasmid construction (mammalian cell produced immunogens or supplied by customer)7-10 weeks
Animal immunizationImmunize two rabbits against the same antigenCrude sera and ELISA data on anti-serum titer test5-8 weeks
B cell isolation and amplificationPBMC collection and cryopreservation
Target positive B cell enrichment and single B cell expansion
100 µl B cell supernatant
B cell supernatant ELISA test data
2-3 weeks
cDNA cloning, sequencing and storageIgG gene cloning
Expression vector construction
Small-scale antibody production and validation
Select 1-2 positive clones for full-length IgG sequencing and sequencing analysis
Sequencing report of one clone
ELISA data
3-4 weeks
Antibody production and validationAntibody production, purification and QC validation1 mg purified recombinant antibody3-4 weeks

Mouse Monoclonal Antibody Development

Mouse is often the first choice when it comes to a therapeutic antibody drug developmental programs. Despite this, mouse monoclonal development comes with many limitations, such as low fusion efficiency of hybridoma and unstable hybridoma cells. Our mouse monoclonal antibody development service overcomes these issues by directly isolating IgG genes from B cells, letting you receive the antibodies you need in record time.

Service Details and Deliverables

StepService DetailsDeliverablesEstimated Production Cycle
Immunogen preparationPlasmid construction (mammalian cell produced immunogens or supplied by customer)7-10 weeks
Animal immunizationImmunize two mice against the same antigenCrude sera and ELISA data on anti-serum titer test8-12 weeks
Collect mouse splenocytesDissect animal and cryopreservation
Target positive B cell enrichment and single B cell expansion
100 µl B cell supernatant
B cell supernatant ELISA test data
4 weeks
cDNA cloning, sequencing and storageIgG gene cloning
Expression vector construction
Small-scale antibody production and validation
Select 1-2 positive clones for full-length IgG sequencing and sequence analysis
Sequencing report of one clone
ELISA data
2 weeks
Antibody production and validationAntibody production and identification
Antibody mass production and purification
Antibody function identification
Antibody production
Purification and QC validation
3-4 weeks

Antibody Development Request Form

Let's get started

Complete our service request form and we will contact you to discuss your requirements.