Spike (B.1.1.529 BA.1/ Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter)
Product Code
AMS.78349-2
$0.00
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The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication/ the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes/ endothelial cells/ and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection. A variant called B.1.1.529 (also known as the Omicron Variant) was identified in South Africa in November of 2021. This variant has a large number of mutations that allow the virus to spread more easily and quickly than other variants.The Spike (B.1.1.529 Variant) (SARS-CoV-2) Pseudotyped Lentiviruses were produced with SARS-CoV-2 B.1.1.529 Variant Spike (Genbank Accession #QHD43416.1 with B.1.1.529 mutations; see below for details) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions contain the eGFP gene driven by a CMV promoter/ therefore/ the spike-mediated cell entry can be determined via eGFP fluorescence. The Spike (B.1.1.529 Variant) (SARS-CoV-2) pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 B.1.1.529 variant in a Biosafety Level 2 facility.The Spike Omicron pseudovirus has been validated for use with target cells ACE2-HEK293 (which overexpress ACE2; / #79951).Spike Mutations in B.1.1.529 Variant:A67V/ ?69-70/ T95I/ G142D/ ?143-145/ ?211/ L212I/ ins214EPE/ G339D/ S371L/ S373P/ S375F/ K417N/ N440K/ G446S/ S477N/ T478K/ E484A/ Q493R/ G496S/ Q498R/ N501Y/ Y505H/ T547K/ D614G/ H655Y/ N679K/ P681H/ N764K/ D796Y/ N856K/ Q954H/ N969K/ L981F
SKU | AMS.78349-2 |
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Size | 500 ul x 2 |
Species | SARS-CoV-2 |
Application | Screening for neutralizing antibodies for the Omicron variant of SARS-CoV-2 Spike in ACE2-HEK293 cells |
Gene synonyms | south africa variant, south africa lenti, India mutation, pseudovirus, pseudovirion, egfp reporter, B11529, 78349-1, 78349-2 |
Storage | Lentiviruses are shipped with dry ice. For long term storage/ it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |