Aptamer Design & Synthesis

Tailor-made aptamers with a high affinity and high specificity for your target molecules

Aptamers are useful in biotechnological and therapeutic applications as they offer molecular recognition properties that rival that of antibodies. In addition to their discriminate recognition, aptamers offer advantages over antibodies as they can be engineered completely in a test tube, are readily produced by chemical synthesis, possess desirable storage properties, and elicit little or no immunogenicity in therapeutic applications. AMSBIO offers a custom service to generate high quality aptamers against your targets, giving you excellent results even with the most difficult target molecules. Our aptamers are fluorescently labeled with various labeling options for convenient visualization.

Our Services

Our custom aptamer service takes advantage of a unique SELEX technology, which allows for the rapid development of aptamers which bind to your target molecules with a high affinity.

Our custom aptamer services comprise:

      • Standard service type and custom research services type
      • Small scale and large scale (grams, µ moles) synthesis

Custom Aptamers (Aptamer Discovery)

      • Advanced SELEX using modified bases
      • Traditional SELEX using non-modified bases
      • Competitive and/or negative selections
      • Maturation SELEX (2nd round SELEX using pre-screened focused library)

Aptamer Modification

      • End group (5’ and/or 3’) modification with/without linker (dT, dA, HEG, PEG etc): Amine, Thiol, Biotin, fluorescent dyes, PC Biotin, PC Amine, idT, Cholesterol, etc.
      • Internal modification: 2’-OMe, internal amine, internal biotin, etc.
      • Conjugation: Pegylation (linear, branched, lysine branched), beads, etc.

Aptamer Characterization

      • Binding assay (using RI method)
      • Competition binding assay
      • Trimming (truncation and optimization)
      • Ligand scan (function study)

Assay Development

      • Cell/Protein isolation (pull down)
      • ELASA/ELISA assay

Schematic Representation

Aptamer Design & Synthesis Request Form

AMSBIO

Complete our service request form and we will contact you to discuss your requirements.