CELLBANKER® Cell Freezing Media
Cryopreservation solution for cells and tissues
The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of cells. With its unique formulation which enables stable cryopreservation and high viability after freeze-thaw procedures, CELLBANKER is a trusted solution for the storage of any cell type including sensitive cell lines.
Available in several formulations, this series of easy-to-use cell freezing media offers high cell viability in serum, serum-free, GMP and DMSO-free formats.
- Enables long term cell storage for >8 years at -80˚C or -196˚C
- Consistent high cell viability (>90%)
- Serum, serum-free and chemically defined formulations
- Ready-to-use formulation with a simple protocol
- Tested on many cells
- No programmed freezer or liquid nitrogen required
- Long shelf life (3 years from manufacturing date)
Serum-containing cryopreservation medium
The first product of the CELLBANKER® series, CELLBANKER® 1, was launched in 1992 and now has a significant history of reliable, consistent and high viability recoveries post-cryopreservation. Contains serum, DMSO, glucose, salts and buffer.
Serum-free cell cryopreservation solution
A serum-free cell freezing medium that allows cell cryopreservation directly at -80°C without requiring a rate controlled freezer. Contains no animal-derived products and is guaranteed sterile.
GMP grade cryopreservation medium optimized for hematopoietic stem cells
HSC-BANKER® is an optimized GMP grade cryopreservation medium for hematopoietic stem cells. Designed with a simple formulation HSC-BANKER® also offers the potential to be used as excipient in cell therapy delivery.
Studies show that the HSC-BANKER® is at least equivalent to conventional protocols using DMSO and DEXTRAN. The results of a comparative study on the cryopreservation of hematopoietic stem cells was performed by the Cord Blood Bank of Japanese Red Cross Society: View Results
CELLBANKER® solutions are simple to use. Achieve the highest cell viability whilst maintaining stem cell pluripotency, normal karyotype and proliferation ability after cryopreservation using CELLBANKER®.
Frequently Asked Questions
What is the difference between CELLBANKER®1, and 2?
CELLBANKER® 1 contains serum; whereas CELLBANKER® 2 is a serum and protein- free type of medium.
What kind of serum is used? Which country does the serum origin?
New Born Calf Serum (NBS) is principally used and its origin is in Australia or New Zealand.
Why is programming freezing not needed for CELLBANKER® cryopreservation?
CELLBANKER® is formulated only for deep- freezer rapid freezing use at approximately -85℃. Freezing procedures by programming freezer or liquid nitrogen may deteriorate the cell viability. Storage of cells in a liquid nitrogen tank is recommended only after a sufficient freeze by deep freezer.
Tell me more about the components? Is dimethylsulfoxide (DMSO) included?
Unfortunately, the components of CELLBANKER® are not available to release at this time. DMSO is used as a frozen protectant in the product. However, DMSO rarely has any effects on the cryopreservation. May we remind you to wash your cells sufficiently after thawing.
Can I preserve my normal cells , lymphocytes or stem cells with CELLBANKER®?
There are some types of cell that are not suitable to be preserved with CELLBANKER®. Therefore, an initial test prior to the actual freezing is recommended.
How can I store CELLBANKER®?
Generally, CELLBANKER® is recommended to be kept at 4℃ and should be completely used as soon as possible. If storing for 3 months or longer, separate into aliquot and keep it frozen (-20℃). To prevent from deterioration of the product, frequent freeze-thaw method (more than 3 times) should be avoided.
Is CELLBANKER® a medicinal product?
CELLBANKER® is for research use only. Therefore, it is prohibited to be use in medical practice.
Immature mDA Neurons Ameliorate Motor Deficits in a 6-OHDA Parkinson's Disease Mouse Model and are Functional after Cryopreservation.
Leitner D et al. (2019) Stem Cell Research, 101617
Derivation of enteric neuron lineages from human pluripotent stem cells.
Barber K et al. (2019) Nature Protocols. Volume 14, 1261–1279
PD-1 signaling modulates interferon-γ production by Gamma Delta (γδ) T-Cells in response to leukemia.
Hoeres T et al. (2019) OncoImmunology. 1869:pp 1–16
Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.
Badodi S et al. (2019) Methods Mol Biol. Volume 8, 2019 - Issue 3
Effect of cryopreservation on the appearance and liver function of hepatocyte-like cells in cultures of cirrhotic liver of biliary atresia.
Yamazaki T et al. (2018) In Vitro Cell Dev Biol Anim. Volume 54, Issue 6, pp 401–405
Exosomes derived from clinical-grade oral mucosal epithelial cell sheets promote wound healing.
Sjöqvist Set al. (2018) Journal of Extracellular Vesicles Volume 8, 2019 - Issue 1
Long-term Cryopreservation of Human and other Mammalian Cells at- 80° C for 8 Years.
Miyamoto. Yet al. (2018) Cell Medicine Volume 10: 1-7
Wang Z et al. (2021) Nature. 592, pages616–622
Generation of Fabry cardiomyopathy model for drug screening using induced pluripotent stem cell-derived cardiomyocytes from a female Fabry patient.
Kuramoto. Y. et al. (2018) Journal of Molecular and Cellular Cardiology Volume 121, Pages 256–26
Fused cerebral organoids model interactions between brain regions.
Bagley. J. A. et al. (2017) Nature Methods. doi:10.1038/nmeth.4304
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.
Yamanaka S et al. (2014) Scientific Reports 4 Article, number:359
Monolayer culturing and cloning of human pluripotent stem cells on laminin-521–based matrices under xeno-free and chemically defined conditions.
Rodin et al. (2014) Nat Protoc. 2014 Oct;9(10):2354-68
Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells
Hata et al. (2014) Nat Protoc. 2014 Oct;9(10):2354-68
Cryopreservation of Induced Pluripotent Stem Cells
Miyamoto Y et al. (2012) Cell Medicine. Vol. 3, pp. 89–95, 2012
An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells.
Holm et al. (2010) Hum Reprod. 25(5):1271-9. Epub 2010 Mar 5.