3D Hydrogels for Advanced Cell Culture
Biologically relevant matrices for 3D cell culture and pdx tumor models
3D hydrogels provide a physiologically relevant environment for cell growth, differentiation, and drug screening, mimicking the native extracellular matrix (ECM). AMSBIO offers two advanced hydrogel solutions—Extragel® and MatriMix™—each optimized for specific applications.
- Extragel® is a like-for-like replacement of industry-standard matrices like Matrigel™, Geltrex™, and Cultrex™ Basement Membrane Extract (BME), with enhanced performance and reproducibility. Its natural ECM like structure has the optimal microenvironment, mimicking native tissue architecture for advanced 2D and 3D cell culture.
- MatriMix™ offers a fully defined alternative, giving researchers precise control over cellular interactions and signaling pathways, and comes in a single vial option for PDX, and a three vial tunable version for 3D Cell Culture.
Together, Extragel® and MatriMix™ represent a significant step forward in 3D cell culture technology, driving forward scientific progress with precision and reliability.
Which Hydrogel is Right for Your Research?
| Feature | Extragel® | MatriMix™ |
|---|---|---|
| Composition | Derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, containing a natural mix of ECM proteins | Defined mixture of Laminin 511E8, Type I Collagen, and Hyaluronic Acid for precise control |
| Applications | General 2D and 3D cell culture, organoid models, tumor spheroids, and stem cell maintenance and differentiation | Patient-Derived Xenograft (PDX) models, tunable 3D cell culture, and customized ECM conditions |
| Batch Consistency | Naturally derived, some lot-to-lot variation | High batch-to-batch reproducibility |
| Customization | Fixed composition | Two versions: Single-vial formulation for PDX models; Three-vial tunable version for adjusting stiffness, adhesion, and signaling factors |
| Protocol Compatibility | Drop-in replacement for Matrigel, making it easy to integrate into existing protocols | Designed for applications requiring defined ECM components and tunability |
| Ethical Considerations | Standard model widely used in research | Uses medical grade porcine collagen, reducing reliance on mouse-derived ECM and supporting the 3Rs principles in scientific research |
Extragel® and MatriMix™ Spotlight
Extragel Matrix: a Like-for-Like replacement for Matrigel™, Geltrex™ and Cultrex™ BME
Extragel is a reconstituted matrix hydrogel formed by basement membrane components. It is mainly composed of laminin, collagen IV, and heparan sulfate proteoglycans as well as various growth factors such as EGF, PDGF, NGF, FGF-2, TGF-β) and IGF.
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- Like-for-like replacement for Matrigel™, Geltrex™, and Cultrex™ BME
- Available immediately with overnight shipping
- Effective growth matrix for organoid, PSCs, and advanced 3D cell culture applications.
- High lot-to-lot consistency
- Suitable for PDX/Xenograft transplantation
MatriMix: A Fully Defined ECM Hydrogel for 3D and PDX
MatriMix™ is an advanced, fully-defined hydrogel containing medical grade collagens, laminin-511 E8 fragments, and hyaluronic acid, engineered for patient-derived xenografts (PDX) and highly controlled 3D cell culture. With a clear gel composition and options of a single vial for PDX, or a tunable three-vial formulation researchers can adjust ECM properties to better match their experimental conditions, MatriMix is a a step forwards.
- High batch-to-batch consistency for reliable, reproducible results
- 3-vial tunable matrix allows control over stiffness, adhesion, and signaling factors for 3D Cell Culture
- Clear gel enhances visualization for microscopy and imaging applications
- Single-vial PDX formulation optimized for tumor engraftment and drug testing
- Regulatory friendly
Find the Right Hydrogel for Your Research
AMSBIO’s 3D ECM Hydrogels offer cutting-edge solutions for researchers working in oncology, drug discovery, and regenerative medicine. Whether you need a natural ECM scaffold (Extragel®) or a tunable, defined hydrogel (MatriMix™), we have the solution for your 3D culture needs.
Need guidance? Contact our specialists for expert recommendations.
