Tools for growth, harvesting & storage
Organoids are organ-like structures that can be formed by 3D cell culture and differentiation of stem cells or organ progenitors, and are capable of recapitulating aspects of organ function in vitro. AMSBIO is launching a suite of products to assist the increasing number of scientists adopting these structures for use in:
AMSBIO supplies a suite of products for organoid growth. Starting with ECM matrices and low-adhesion plates to provide the right microenvironment, we can also provide many of the necessary cues and growth factors (notably Wnt3a, R-spondin 1) to help your stem or progenitor cells follow the correct developmental pathway to differentiate into your organoid of choice.
Recombinant human and mouse Wnt3A are both available from AMSBIO. The combination of Wnt3A and the Wnt amplifier R-spondin1 is essential to grow organoids from normal epithelium.
Your Wnt3a in the TOP/FOP Flash Reporter Assay showed higher activity at the same concentration than the industry leader
The Lipidure®-coated plates provided by AMSBIO were extremely useful for generating with ease large numbers of homogeneous retinal organoids which responded to light and contained all the key retinal cell types
FACSmax™ Cell Dissociation Solution
Single cells from organoids can be harvested using FACSmaxTM Cell Dissociation Solution which is ready to use, gentle and highly effective solution causing disaggregation of cells while preserving maximum cell viability at the same time.
Leptin and adiponectin modulate the self-renewal of normal human breast epithelial stem cells.
Esper, R. M., Dame, M., McClintock, S., Holt, P. R., Dannenberg, A. J., Wicha, M. S., & Brenner, D. E. (2015). Cancer Prevention Research, 8(12), 1174-1183.
Dietary polyunsaturated fatty acids modulates adipose Secretome and is associated with changes in mammary epithelial stem cell self-renewal.
Hill, E. M., Esper, R. M., Sen, A., Simon, B. R., Aslam, M. N., Jiang, Y., ... & Brenner, D. E. (2019). The Journal of Nutritional Biochemistry.
CELLBANKER® Freezing Media Series
CELLBANKER® is a series of easy-to-use cell cryopreservation media. CELLBANKER® enables long term storage of different cell types; maintaining consistent high cell viability regardless of their sensitivity, due to superior protection against cell stress during freeze/thaw cycles. CELLBANKER® media have been validated for cryopreservation of whole organoids, and also for archiving clones with desired genetic characteristics that can later be grown into organoids
As a cell freezing medium, CELLBANKER® does not require a gradual temperature decrease in a programmed freezer or storage in liquid nitrogen to guarantee efficient cell storage. This makes CELLBANKER® more affordable and accessible than other cell freezing media. CELLBANKER® solutions are simple to use and allow you to achieve the highest cell viability while maintaining your cells’ natural functions. Read the full protocol for organoid cryopreservation
Day 2 and day 4 images clearly show that organoids recovered and grew well in these three CELLBANKER® freezing media
Cryopreservation of cholangiocyte organoid (CO) pellets with CellBanker 2 (Amsbio, cat. no. 11891):
Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue
Tysoe, O. C., Justin, A. W., Brevini, T., Chen, S. E., Mahbubani, K. T., Frank, A. K., ... & Vallier, L. (2019). Nature Protocols, 14(6), 1884.
The Knoblich lab in Vienna (pioneers in brain organoid culture) cited CellBanker 2 for archiving clones with desired genetic characteristics that can later be grown into organoids:
Fused cerebral organoids model interactions between brain regions.
Bagley, J. A., Reumann, D., Bian, S., Lévi-Strauss, J., & Knoblich, J. A. (2017). Nature Methods, 14, 743.