StemFit® Feeder-Free Stem Cell Culture Media

Allowing weekend-free culture of stem cells

StemFit® is a range of xeno-free, chemically defined media proven to effectively maintain induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) under feeder-free conditions during the reprogramming, expansion and differentiation phases of stem cell culture.

Available in research and clinical grade formulations, StemFit® combines market-leading colony forming efficiency with lower than standard media volume consumption to offer the most cost-effective colony expansion compared to leading competitors.

  • Xeno-free & feeder-free
  • Weekend-free cell culture
  • Reduced media consumption
  • Reproducible growth rates
  • Easy transition from feeder cells
  • Superior colony forming efficiency from a single clone
  • Compatible with a large number of matrices

StemFit® Basic03 has been used together with iMatrix-511 recombinant laminin E8 in a world-first clinical study of iPSC-derived corneal epithelial cells by Professor Ryuhei Hayashi and co-workers from the Graduate School of Medicine, Osaka University, Japan. See more details and an interview here.

The StemFit® Range

Basic02

A xeno-free, chemically defined medium for the culture of pluripotent stem cells.

Basic03

A defined medium for pluripotent stem cell culture. Free of human and animal material, suitable for clinical research.

Basic04

The newest StemFit® formula. A chemically defined medium (free of human and animal material) for both basic and clinical research.

Basic04 Complete

Including bFGF at 80 ng/ml. Chemically defined and free of human and animal material. Suitable for basic and clinical research.

FeatureBasic02Basic03Basic04Basic04 Complete
Suitable for Single-cell CultureYesYesYesYes
Free of Animal & Human MaterialXeno Free*YesYesYes
cGMP-Available†In DevelopmentIn Development
Basic ResearchYes-YesYes
Clinical Research-YesYesYes
Number of Bottles2211
bFGFSold SeparatelySold SeparatelyPromotion Sample Provided‡Included (80ng/ml)

*Contains material derived from human plasma
SFB-503-GMP: Made in USA under US cGMP
One sample vial of bFGF per bottle of StemFit Basic04. Promotion until December 2020

Related Products

NamePacksize Order
Human recombinant Activin A10 ug View
Human recombinant Activin A50 ug View
Human recombinant FGF Basic1 mg View

Get 50% More For The Same Cost

See how StemFit® provides more wells than any other leading competitor product.

StemFit® For Differentiation

StemFit® For Differentiation is a chemically defined and human/animal component-free supplement for differentiation of human ES and iPS cells to multiple lineages. It can be used with a variety of different induction factors or cytokines to support differentiation along ectoderm, mesoderm or endoderm lineages.

StemFit® For Differentiation can be combined with StemFit® medium for human PSCs, to support clinical research applications of hPSC-derived cells/tissues by providing defined and xeno-free culture systems for both expansion and differentiation. StemFit® For Differentiation is provided as 5x concentrate and is intended to be used with basal medium (DMEM, DMEM/F12, RPMI1640, etc.) and appropriate induction factors or cytokines.

NameDatasheetPacksize Order
StemFit® For Differentiation100 ml View

Weekend-free Cell Culture

The maintenance of stem cells is a complicated and labor-intensive process, requiring a tedious feeding step at the weekends. StemFit® frees up your weekends - simply use the recommended weekly workflow shown below to reduce workload and save time!

Reduced Media Consumption

Due to the high-quality components and the ideal concentration of nutrients, the required volume of StemFit® is lower than that of conventional media. Additionally, reduced feeding steps during the week, creates a total volume difference between StemFit® and conventional media of more than 50%.

Conventional Protocol vs StemFit protocol

The volume of StemFit® can be reduced by 25% per well. The reduction in number of media changes per week leads to a further volume reduction of more than 50%.

Reproducible Growth Rate

No more variation due to feeder cells! Cultivating stem cells using StemFit® results in a very reproducible growth rate, meaning you can plan your experiments better. Not only that, analyzing the morphology of stem cells cultivated in StemFit® shows that the colony shape and size are very similar to the cells grown on feeder cells.

Total Fold Expansion
Colony Morphology
Colony Morphology

Human 201B7 iPSCs were cultured on iMatrix Laminin-511 with StemFit® for 4 weeks without weekend feeding. Cell colonies were dissociated into single cells and seeded at the listed densities.

Easy Transition from Feeder Cells

The transition from feeder cell conditions to feeder-free could not be easier with StemFit®. Simply switch the medium two days before passaging cells and continue cultivating them in StemFit®. After 2-3 passages the feeder cells will be completely diluted out and you will be left with a pure stem cell culture.

Start From A Single Clone

StemFit® culture medium allows for a superior colony forming efficiency from a single cell clone, which minimizes the effects of stress and results in reliable cells for downstream applications.

Greater than 100x Fold Expansion

StemFit® has been tested on many different matrices. There is a greater than 100 fold increase in the expansion rate of cells cultured in StemFit® when compared to Medium A from the market leading competitor. Different matrices were used as a growing matrix and combined with StemFit® or with Medium A.

Consistent Gene Expression

Typical methods of stem cell cultivation can subject cells to a lot of unnecessary stress. Unwanted changes in the gene expression profile can be introduced during every passage or growth period. Because of this, the Cell and Gene Therapy Catapult (CGT Catapult) in London investigated the genomic profile of cells cultured in StemFit® compared to 4 commercially available media. The most consistent gene expression was obtained using StemFit®. For further details, refer to Superior Performance of StemFit for the Culture of Induced Pluripotent Stem Cells, an independent study performed by CGT Catapult.

Less Lactate Production

Hypoxic stress can result in the production of lactate which can lead to changes in the genome expression profile or unwanted differentiation of stem cells. The CGT Catapult showed that there is considerably less lactate production when the cells are grown in StemFit®.

Featured Citations

Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids
Gupta, A. K., Sarkar, P., Wertheim, J. A., Pan, X., Carroll, T. J., & Oxburgh, L. (2020) Communications Biology 3(1), 1-11.

Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
Ryuji Morizane & Joseph V Bonventre (2017) Nature Protocols 12, 195–207.

Multilineage communication regulates human liver bud development from pluripotency
Camp, J. G. et al. (2017). Nature 2017 Jun 22;546(7659):533-538.

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells
Nakagawa, M. et al.(2014). Scientific Reports. 4, Article number: 3594.