Lipidure®-COAT Plates

Low Adhesion Spheroid and Organoid Culture

Lipidure®-COAT plates are one of the most effective tools for state-of-the-art 3D spheroid and EB cell culture. There are also papers reporting usage of Lipidure®-COAT plates to differentiate EBs into organoids. The Lipidure® coating provides a superior low-attachment for the formation of single spheroids in each well of multi-well plates.

Benefits

  • A superior low-attachment solution
  • Easy to handle
  • Excellent reproducible results
  • Compatible with variety of cell-based assays

Spheroid and embryoid body (EB) cell culture is typically based on the spontaneous formation of an aggregate of cells in an environment where cell-cell interactions dominate over cell-substrate interaction. This can be achieved by using low-attachment cell culture conditions.

Lipidure® powder is a biocompatible and hydrophilic white powder which consists of 2-(methacryloyoxy)ethyl 2-(trimethylammonio)ethyl phosphate-n-butylmethacrylate copolymer. The building unit of this copolymer is 2-(methacryloyoxy)ethyl phosphorylcholine (MPC) monomer. Lipidure® mimics cell membrane surface and its molecular structure is the key for high hydrophilic nature and extremely low toxicity.

How Does Lipidure®-COAT Work?

What Is Lipidure
  1. Low-adhesion surface promotes cell aggregation & spheroid formation.
  2. Uses a biocompatible MPC Polymer containing Phosphoryl Choline (which is found in cell membranes).
  3. Completely synthetic, containing no substances of biological origin.

Formation of Single, Centrally Located Spheroid/EB on U-Bottom Plate

Cell Types Tested

Spheroid formation using Lipidure® has already been demonstrated for the following cell types:

  • human and mouse ES cells
  • human and mouse iPS cells
  • NIH3T3
  • pre-adipocytes
  • HepG2 and other cancer cell lines

Applications

Choose your well shape on Lipidure®-Coat Plates

Overall morphology of organoids in a culture can be altered significantly by whether cells are reaggregated in a V-bottom or U-bottom well (see Mellough et al 2019).

Superior Spheroid Formation

A single spheroid is generated in the Lipidure®-COAT U-bottom well, while numerous satellite spheroids are found in the competitor’s plates. This phenomenon shows that a lot of cells adhered to the competitor’s “low-adhesion” surface.

Seeding Number (cells/well)Diameter of Spheroid (μm)
Lipidure-COATCompetitor
1,000185 ± 12161 ± 9
10,000423 ± 7323 ± 46

Organoid Culture

Images courtesy of Dr. Valeria Chichagova (Newcells Biotech) and Prof. Lako (Newcastle University).

Retinal organoids continuously grown on Lipidure-coated plates from the start of differentiation. Immunofluorescence images of 150 day old organoids show rod and cone photoreceptors containing outer segments on the apical side and synaptic terminals at the base. Muller glial cells span across the entire thickness of retinal neuroepithelium.

“The Lipidure-coated plates provided by AMSBIO were extremely useful for generating with ease large numbers of homogeneous retinal organoids which responded to light and contained all the key retinal cell types.”

Prof. Majlinda Lako, Newcastle University

Day 2: Uniform spherical aggregates
Day 4: Ruffling, indicating formation of vesicles

Fig. Aggregation and early organoid development of inner ear organoids on U-bottom Lipidure®-Coat Wells – courtesy of the Hashino lab, Indiana University School of Medicine

Spheroid Formation and Application in Anti-Cancer Drug Tests Using Lipidure®-COAT

Low Cell Binding Property

Embryoid Body Formation

Neurosphere Formation

Product List

NameDatasheetPacksize Order
Lipidure-CM5206 (White powder), 100g100 g View
Lipidure-CM5206 (White powder), 10g10 g View
Lipidure-CM5206 (White powder), 1g1 g View
Lipidure-Coat Low Adhesion Plate A-U96 (96 well U-bottom plate), 6 plates/box-6 plates View
Lipidure-Coat Low Adhesion Plate A-V96 (96 well Conical V-bottom plate), 6 plates/case-6 plates View

Contact us to ask about our  96-well flat-bottom Lipidure®-Coat plates

Featured Citations

Self-organizing optic-cup morphogenesis in three-dimensional culture.
Eiraku, M. et al. Nature, 2011, 472 (7341), 51. Highly cited! (over 1 thousand times) - U bottom plates

Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences. 
Mellough, C. B., Collin, J., Queen, R., Hilgen, G., Dorgau, B., Zerti, D., ... & Lako, M. (2019).  Stem cells translational medicineU and V bottom plates

Retinal organoids from pluripotent stem cells efficiently recapitulate retinogenesis. 
Völkner, M. et al.  Stem Cell Reports, 2016, 6 (4), 525-538. U bottom plates

 The Ca2+/Mn2+-transporting SPCA2 pump is regulated by oxygen and cell density in colon cancer cells. 
Jenkins, J. et al.Biochemical Journal, 2016, 473 (16), 2507-2518. U bottom plates

Investigation of brain tissue infiltration by medulloblastoma cells in an ex vivo model. 
Neve, A. et al. Scientific reports, 2017, 7 (1), 5297. U bottom plates

Antineoplastic effect of 1α, 25 (OH) 2D3 in spheroids from endothelial cells transformed by Kaposi’s sarcoma-associated herpesvirus G protein coupled receptor. 
Suares, A. et al. The Journal of steroid biochemistry and molecular biology, 2019, 186, 122-129. Flat bottom plates