Features

  • Ideal for feeder-free cell culture
  • Provides superior adhesion of human ES and iPS cells
  • Enables the passaging of single cells
  • Compatible across a wide variety of cell types
  • Makes it easy to achieve extended cultures of hES/hiPS cells
  • Enables efficient bulk production

Why the E8 Fragment?

There are more than 12 Laminin isoforms, all of which promote stem cell growth. Laminin-511 is a major component of the basement membrane, which is expressed in early development of the embryo and can be used as a matrix for pluripotent (ES/iPS) stem cells, as it binds to integrin on cell surfaces. Laminin-511 is more efficient at enhancing cell growth than other Laminins such as Laminin-111 (Matrigel). However, Laminin-511 is a large protein (800kDa) composed of three chains (alpha, beta and gamma), making it difficult to produce recombinantly. In order to overcome this challenge, Laminin-511 proteins were fragmented to find the smallest integrin-binding component and hES cells were found to adhere more strongly to the E8 fragment than to the full-length protein.

Culture More ES & iPS Cells

Figure 2 results show that the number of cells multiplied by 200 or more with the use of Laminin-511 E8 fragment in comparison with the conventional method (colony method).

Superior Binding Activity

The Binding activity of Laminin 511 E8 Fragment against ES cell was better than Full length 511 and traditional substrate. The same function was confirmed in human iPS cells.

iMatrix-511

For maintenance and expansion of Pluripotent Stem Cells

A highly purified and refined product of human recombinant laminin-511 E8 fragment expressed by CHO-S cells. iMatrix-511 easily enables extended culture of human pluripotent stem cells and has swiftly established itself as the gold standard for stem cell culture.

The clinical grade version of iMatrix-511 is also available; iMatrix-511 MG meets the Standards of Biological Ingredients set by the PMDA, a Japanese government agency homologous to the FDA. To enquire about iMatrix™-511 MG, please email us at [email protected]

NameDatasheetPacksize Order
Recombinant Laminin iMatrix-5111050 ug (6 x 175 ug tubes) View
Recombinant Laminin iMatrix-511350 ug (2 x 175 ug tubes) View

iMatrix-511 Silk

For maintenance and expansion of Pluripotent Stem Cells

iMatrix-511 Silk is human recombinant Laminin-511 E8 fragment with the same functions and performance as those of iMatrix-511, the only difference being is that it is expressed in transgenic silkworm cocoon rather than CHO-S cells. The highly productive transgenic silkworms make recombinant laminin fragment at a competitive price.

NameDatasheetPacksize Order
Recombinant Laminin iMatrix-511 silk1050 ug (6 x 175 ug tubes) View

Additional iMatrix™ products

iMatrix-411

For induction of vascular endothelial cells from hPSCs

Laminin-411 (α4β1γ1) is the major isoform that lines the basement membrane of endothelial cells in capillary vessels and binds mainly to the cell surface transmembrane receptors integrin α6β1. iMatrix-411 contains the essential laminin-411 E8 fragment to efficiently induce vascular endothelial cells from hPSCs/iPSCs.

NameDatasheetPacksize Order
Recombinant Laminin iMatrix-4111050 ug (6 x 175 ug tubes) View
Recombinant Laminin iMatrix-411350 ug (2 x 175 ug tubes) View

iMatrix-221

For enrichment and maintenance of cardiomyocytes

Laminin 221 (α2β2γ1) is abundantly present in the basement membrane of muscle tissues such as cardiac muscle and skeletal muscle. It is thought to be involved in muscle cell differentiation and functional maintenance.

Laminin 221 (and laminin 211) are known to bind to integrin α7X2β1 protein, which is selectively expressed in muscle tissue, in particular, in cardiac muscle cells and skeletal muscle cells.

NameDatasheetPacksize Order
Recombinant Laminin iMatrix-221-1050 ug (6 x 175 ug tubes) View
Recombinant Laminin iMatrix-221350 ug (2 x 175 ug tubes) View

Coming soon

iMatrix-111 (α1β1γ1) - major substrate for nerve axon growth, both in vivo and in vitro

iMatrix-332 (α3Aβ3γ2) - essential for epithelial cell adhesion to the basement membrane

Register your interest by contacting us at [email protected]

iMatrix-511 Frequently Asked Questions

What format(s) are the iMatrix™ products sold in?
  • Currently, it is sold only in liquid form. One tube contains 175 ug of cell culture substrate (laminin 511-E8 fragment) at a concentration of 0.5 mg / mL.
  • The lyophilized version of iMatrixTM-511 has been discontinued by the manufacturer as of March 2015.
What is the product storage temperature and expiration date?
  • The storage temperature of the product is refrigerated at 2-8 ° C
  • Please refer to the table below for product expiration date.
Expiration date Applicable products
2 years from date of production iMatrixTM-511, iMatrixTM-511 silk, iMatrixTM-511MG, iMatrixTM-411, iMatrixTM-221
1 year and 6 months from date of production Easy iMatrixTM-511, Easy iMatrixTM-511 silk
6 months from date of production Quick iMatrixTM-511, Quick iMatrixTM-511 silk

[ The specific expiration date is printed on the product box. ]

Is it possible to store iMatrix™ frozen? (-80 °C)
  • No. It can not be stored frozen.
What is the purity of the iMatrix™ products?
  • Purity is over 95%. Please refer to the lot specific data sheet for more information.
What is the recommended medium for culturing hES/hiPS cells when using iMatrix™ products?
  • Miyazaki et al. Nature communications3(1236), 1-10, 2012, and Scientific Reports7, 41165, 2017.
  • The following are used.
    ・mTeSR1,TeSR2,TeSR-E8 (STEMCELL Technologies)
    ・StemProhESCSFM (Thermo Fisher Scientific)
    ・StemFitAK03 (Ajinomoto)
  • Nakagawa et al. Scientific Reports4(3594), 1-7, 2014.
    The following are used.
    ・StemFit (Ajinomoto)
  • The publication stated that all media showed good results.
What is the optimal coating concentration for iPS cell culture?
  • The optimal coating concentration depends on the cell line and must be determined by the end user. We recommend first trying iMatrixTM at 0.5 ug / cm2 and consider the optimal concentration to be between 0.1 and 1.5 ug / cm2.
  • In addition, there is also a new method for plating, the Pre-mix method:
    Miyazaki et al. Scientific Reports7, 41165, 2017.
Is there a culture protocol for iPS cells using iMatrix™-511?
  • Please refer to the link below for the expansion culture protocol of ES / iPS cells using iMatrixTM-511 and the movie of passage operation.

(Expansion culture protocol)
(Movie of passage operation)

Is Rock Inhibitor (Y-27632) necessary for culturing iPS cells?
  • Nakagawa et al. Scientific Reports4(3594), 2014.
  • Use Rock Inhibitor at cell passage. However, it is not used during medium exchange.
Can iPS cell passage be handled with a single cell?
What is used to detached the cells?
Can this product be used for mouse iPS cells?
  • Currently, there is no data to support culture of mouse iPS cells using iMatrixTM-511.
What is the difference between iMatrix™-511 and Matrigel?
  • Matrigel contains laminin-111 from mouse EHS sarcoma. It also contains molecules other than laminin.
  • iMatrixTM-511 is a recombinant protein purified from laminin 511-E8 fragment expressed in CHO-S cells with high purity.
  • iMatrixTM-511 silk is a recombinant protein in which the laminin 511-E8 fragment is highly purified by silkworm-produced cocoons.
  • Human ES cells and iPS cells are known to adhere to laminin-511 through cell membrane receptors (especially a6b1 integrin).
  • Human ES cells and iPS cells are known to exhibit high adhesion activity to laminin-511.
    This makes it possible to pass iPS cells into single cells and pass them with iMatrixTM-511 / iMatrixTM-511 silk.
  • In Miyazaki et al. (Nature communications, 3 (1236), 1-10, 2012), the expansion efficiency of the number of cells after 5 passages (after 30 days) is about 200 times higher than that of Matrigel.

Stem Cell Synergy

AMSBIO's iMatrix laminin can be used together with our StemFit cell culture media and CELLBANKER series cryopreservation solution, to create the perfect Stem Cell Synergy for your ES/iPSC cell culture!