CRISPR/Cas9 Genome Editing

Cutting edge tools for your genome editing research

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is the latest tool in genome editing. A simple yet powerful technology, CRISPR/Cas9 has been adapted from a natural defense mechanism of bacteria and archaea, and can be utilized to target specific sites in the genome. This revolutionary technology allows for the manipulation of specific genes with high specificity and low cell toxicity.

AMSBIO supplies an extensive range of genome editing products to support your research.

How does CRISPR/Cas9 work?

The first step is to design a guide RNA which is complementary to your target gene, and inject into the cell you wish to modify along with a Cas9 protein. Cas9 is a CRISPR-associated endonuclease and acts as a pair of molecular scissors by cutting the target DNA in the specific desired location.

The guide RNA and the Cas9 form a complex, and the Cas9 is guided to the target gene, where the enzyme unwinds the DNA duplex and cleaves both strands specifically at this site. Target recognition can only occur in the presence of a protospacer adjacent motif (PAM) which flanks the target site.

By harnessing the cell’s natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from, leading to targeted insertions. CRISPR/Cas9 technology therefore has the potential to revolutionize the way in which we treat disease, as well as a whole host of other applications, offering numerous advantages over other gene editing methods.

Our Products

NameDatasheetPacksize Order
Cas9 Nickase: EF1-T7-hspCas9-nickase-T2A-GFP-H1-gRNA linearized SmartNickase vector10 reactions View
Cas9 SmartNuclease AAVS1-gRNA Targeting Vector [EF1a promoter]-10 ug View
EF1-T7-hspCas9-T2A-GFP-H1-gRNA linearized SmartNuclease vector-10 reactions View
EF1a-hsaCas9-U6-gRNA(SA) linearized all-in-one SmartNuclease AAV Plasmid-10 reactions View
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector-10 reactions View
Precut pCAS-Guide cloning kit (10 RXNs), GE100007 and GE100008 included.1 kit View
Precut pLenti-Cas-Guide cloning kit (10 RXNs), GE100007 and GE100008 included.1 kit View
pCas-Guide CRISPR Vector1 kit View
pCas-Guide-EF1a-CD4 CRISPR Vector-1 kit View
pCas-Guide-EF1a-GFP CRISPR Vector-1 kit View
pCas-Scramble CRISPR Vector1 kit View
pCas-Scramble-EF1a-GFP CRISPR Vector-1 kit View
pLenti-Cas-Guide CRISPR Vector1 kit View
pLenti-EF1a-Cas-Guide CRISPR Vector-1 kit View

NameDatasheetPacksize Order
pAAVS1-Cas9-BSD-DNR CRISPR Vector-1 kit View
pAAVS1-Cas9-Puro-DNR CRISPR Vector-1 kit View
pGuide CRISPR Vector-1 kit View
pGuide-EF1a-GFP CRISPR Vector-1 kit View
pLenti-Cas9 CRISPR Vector-1 kit View
pLenti-Cas9-IRES-Puro CRISPR Vector-1 kit View
pLenti-Cas9-P2A-tGFP CRISPR Vector-1 kit View
pLenti-Guide-Puro CRISPR Vector-1 kit View
pT7-Cas9 CRISPR Vector1 kit View

NamePacksize Order
CAS9 Streptococcus pyogenes Recombinant Protein500 pmol View

NamePacksize Order
pGuide CRISPR Vector1 kit View
pGuide-EF1a-GFP CRISPR Vector1 kit View
pLenti-Guide-Puro CRISPR Vector1 kit View
pT7-Guide-IVT CRISPR Vector1 kit View

NameDatasheetPacksize Order
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)10 ug View
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)-1 kit View
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)-10 ug View
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)1 kit View
AAVS1 Transgene Knockin kit (BSD) CRISPR Vector-1 kit View
AAVS1 Transgene Knockin kit (EF1a, Puro) CRISPR Vector-1 kit View
AAVS1 Transgene knockin vector kit CRISPR Vector-1 kit View
Cas9 SmartNuclease AAVS1-gRNA Targeting Vector [EF1a promoter]-10 ug View
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)-10 ug View
pAAVS1-BSD-DNR CRISPR Vector-1 kit View
pAAVS1-EF1a-BSD-DNR CRISPR Vector-1 kit View
pAAVS1-EF1a-puro-DNR CRISPR Vector-1 kit View
pAAVS1-Puro-DNR CRISPR Vector-1 kit View
pCas-Guide-AAVS1 CRISPR Vector-1 kit View

NamePacksize Order
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version)20 ug View
Transfection-ready Cas9 SmartNuclease mRNA (Prokaryotic version)20 ug View

NamePacksize Order
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector10 reactions View
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector10 reactions View
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector10 reactions View
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector10 reactions View
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector10 reactions View
PrecisionX Multiplex gRNA Cloning Kit10 reactions View

NamePacksize Order
pCas-Guide-Nickase (D10A) CRISPR Vector1 kit View
pT7-Cas9-Nickase (D10A) CRISPR Vector1 kit View

NameDatasheetPacksize Order
AAVS1 Safe Harbor Positive Control Donor Vector [pAAVS1D-CMV.RFP-EF1a.copGFPpuro]-10 ug View
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS)10 ug View
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS)-10 ug View
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP)-10 ug View
AAVS1 Safe Harbor cDNA/miRNA Donor Vector [pAAVS1D-PGK.MCS-EF1a.copGFPpuro]-10 ug View
Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out-10 ug View
GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]-10 ug View
GFP-Fusion HR Targeting Vector [GFP-pA-LoxP-EF1a-RFP-T2A-Hygro-pA-LoxP-MCS]-10 ug View
GFP-T2A-Luciferase Co-Expression HR Targeting Vector [GFP-T2A-Luc-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]-10 ug View
Gene Knock-Out HR Targeting Vector [MCS1-EF1?-RFP-T2A-Puro-pA-MCS2]10 ug View
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]-10 ug View
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2]-10 ug View
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration10 ug View
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration-10 ug View
Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration-10 ug View
Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]-10 ug View
IRES-GFP Co-Expression HR Targeting Vector [IRES-GFP-pA-loxP-MCS1-EF1?-RFP-T2A-Puro-pA-LoxP-MCS2]-10 ug View
T2A-GFP Co-Expression HR Targeting Vector [T2A-GFP-pA-loxP-EF1?-RFP-T2A-Puro-pA-LoxP-MCS]-10 ug View
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)-10 ug View

Can't find what you're looking for?

We offer a range of CRISPR custom services for your genome editing research