CRISPR/Cas9 Genome Editing Products
Cutting edge tools for your genome editing products
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is the latest tool in genome editing. A simple yet powerful technology, CRISPR/Cas9 has been adapted from a natural defense mechanism of bacteria and archaea, and can be utilized to target specific sites in the genome. This revolutionary technology allows for the manipulation of specific genes with high specificity and low cell toxicity.
AMSBIO supplies an extensive range of genome editing products to support your research.
How does CRISPR/Cas9 work?
The first step is to design a guide RNA which is complementary to your target gene, and inject into the cell you wish to modify along with a Cas9 protein. Cas9 is a CRISPR-associated endonuclease and acts as a pair of molecular scissors by cutting the target DNA in the specific desired location.
The guide RNA and the Cas9 form a complex, and the Cas9 is guided to the target gene, where the enzyme unwinds the DNA duplex and cleaves both strands specifically at this site. Target recognition can only occur in the presence of a protospacer adjacent motif (PAM) which flanks the target site.
By harnessing the cell’s natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from, leading to targeted insertions. CRISPR/Cas9 technology therefore has the potential to revolutionize the way in which we treat disease, as well as a whole host of other applications, offering numerous advantages over other gene editing methods.
|AAVS1 Transgene Knockin kit (BSD)||-||1 kit||View|
|AAVS1 Transgene Knockin kit (EF1a, Puro)||1 kit||View|
|AAVS1 Transgene knockin vector kit||-||1 kit||View|
|pAAVS1-EF1a-puro-DNR CRISPR Vector||10 µg||View|
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|CRISPRa SAM Vector Kit, CRISPR/Cas9 Gene Activation||1 kit||View|
|pCRISPRa-Enhancer Vector, Synergistic Transactivation Domains for CRISPRa||10 µg||View|
|pCas-Guide-CRISPRa gene activation vector||10 µg||View|
|pCas-Guide-CRISPRi vector|All-in-one Vector for CRISPR/Cas9 Gene Interference with dCas9-KRAB-MeCP2 and gRNA cloning site.||10 Ã‚Âµg||View|
|pCas-Guide-GFP-CRISPRa gene activation vector||10 µg||View|
|pCas-Guide-GFP-CRISPRi gene interference vector||10 µg||View|
|pCas-Guide-Puro-CRISPRa gene activation vector||10 µg||View|
|pCas-Guide-Puro-CRISPRi Vector|All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker|dCas9-KRAB-MeCP2 and gRNA cloning site.||10 Ã‚Âµg||View|