CRISPR/Cas9 Genome Editing

Cutting edge tools for your genome editing research

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is the latest tool in genome editing. A simple yet powerful technology, CRISPR/Cas9 has been adapted from a natural defense mechanism of bacteria and archaea, and can be utilized to target specific sites in the genome. This revolutionary technology allows for the manipulation of specific genes with high specificity and low cell toxicity.

AMSBIO supplies an extensive range of genome editing products to support your research.

How does CRISPR/Cas9 work?

The first step is to design a guide RNA which is complementary to your target gene, and inject into the cell you wish to modify along with a Cas9 protein. Cas9 is a CRISPR-associated endonuclease and acts as a pair of molecular scissors by cutting the target DNA in the specific desired location.

The guide RNA and the Cas9 form a complex, and the Cas9 is guided to the target gene, where the enzyme unwinds the DNA duplex and cleaves both strands specifically at this site. Target recognition can only occur in the presence of a protospacer adjacent motif (PAM) which flanks the target site.

By harnessing the cell’s natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from, leading to targeted insertions. CRISPR/Cas9 technology therefore has the potential to revolutionize the way in which we treat disease, as well as a whole host of other applications, offering numerous advantages over other gene editing methods.

Our Products

NameDatasheetPacksize Order
Precut pCAS-Guide cloning kit (10 RXNs), GE100007 and GE100008 included.1 kit View
Precut pLenti-Cas-Guide cloning kit (10 RXNs), GE100007 and GE100008 included.1 kit View
pCas-Guide CRISPR Vector1 kit View
pCas-Guide-EF1a-CD4 CRISPR Vector-1 kit View
pCas-Guide-EF1a-GFP CRISPR Vector-1 kit View
pCas-Scramble CRISPR Vector1 kit View
pCas-Scramble-EF1a-GFP CRISPR Vector-1 kit View
pLenti-Cas-Guide CRISPR Vector1 kit View
pLenti-EF1a-Cas-Guide CRISPR Vector-1 kit View

NameDatasheetPacksize Order
pAAVS1-Cas9-BSD-DNR CRISPR Vector-1 kit View
pAAVS1-Cas9-Puro-DNR CRISPR Vector-1 kit View
pGuide CRISPR Vector-1 kit View
pGuide-EF1a-GFP CRISPR Vector-1 kit View
pLenti-Cas9 CRISPR Vector-1 kit View
pLenti-Cas9-IRES-Puro CRISPR Vector-1 kit View
pLenti-Cas9-P2A-tGFP CRISPR Vector-1 kit View
pLenti-Guide-Puro CRISPR Vector-1 kit View
pT7-Cas9 CRISPR Vector1 kit View

NamePacksize Order
CAS9 Streptococcus pyogenes Recombinant Protein500 pmol View

NamePacksize Order
pGuide CRISPR Vector1 kit View
pGuide-EF1a-GFP CRISPR Vector1 kit View
pLenti-Guide-Puro CRISPR Vector1 kit View
pT7-Guide-IVT CRISPR Vector1 kit View

NamePacksize Order
AAVS1 Transgene Knockin kit (BSD) CRISPR Vector1 kit View
AAVS1 Transgene Knockin kit (EF1a, Puro) CRISPR Vector1 kit View
AAVS1 Transgene knockin vector kit CRISPR Vector1 kit View
pAAVS1-BSD-DNR CRISPR Vector1 kit View
pAAVS1-EF1a-BSD-DNR CRISPR Vector1 kit View
pAAVS1-EF1a-puro-DNR CRISPR Vector1 kit View
pAAVS1-Puro-DNR CRISPR Vector1 kit View
pCas-Guide-AAVS1 CRISPR Vector1 kit View

NamePacksize Order
We couldn't find any records.

NamePacksize Order
We couldn't find any records.

NamePacksize Order
pCas-Guide-Nickase (D10A) CRISPR Vector1 kit View
pT7-Cas9-Nickase (D10A) CRISPR Vector1 kit View

NamePacksize Order
We couldn't find any records.

Can't find what you're looking for?

We offer a range of CRISPR custom services for your genome editing research