NASBA Kits & Reagents

Easily amplify and detect RNA with our NASBA kits

Reverse transcriptase PCR (RT-PCR) is currently one of the most commonly used techniques for performing RNA detection, although it suffers from false positives due to cross-contamination whilst being a time consuming process. A more convenient technique for RNA detection is Nucleic Acid Sequence Based Amplification (NASBA), which is a one-step isothermal process for amplifying RNA. NASBA has demonstrated success in the detection of viral (including SARS-CoV-2) and bacterial RNA from clinical samples.

Each NASBA reaction contains avian myeloblastosis virus-reverse transcriptase (AMV-RT), T7 RNA polymerase and RNase H with reaction buffer. Due to NASBA being an isothermal process, denaturation of dsDNA does not occur and ssRNA can be amplified. Therefore, false negatives for the NASBA reaction are prevented that would otherwise be caused by genomic dsDNA in other techniques like RT-PCR.

NASBA Kits

We offer a range of NASBA kits for RNA detection, which are designed as single product solutions for any NASBA related research and development. The components of the kit are available either in liquid form or lyophilized form to allow shipping and storage at standard room temperature.

NameDatasheetPacksize Order
3X NASBA Reaction Buffer24 reactions View
NASBA Enzyme Cocktail (wet mix)24 reactions View
NASBA Lyophilized Kit (10 reactions)10 reactions View
NASBA Lyophilized Kit (5 reactions)5 Reactons View
NASBA Wet Kit24 reactions View

AMV-RT

We also provide AMV-RT as a standalone product for purchase, available in glycerol storage buffer in addition to Trehalose storage buffer (suitable for lyophilization):

NameDatasheetPacksize Order
AMV Reverse Transcriptase1000 U View
AMV Reverse Transcriptase25000 U View
AMV Reverse Transcriptase300 U View
AMV Reverse Transcriptase-5000 U View
AMV Reverse Transcriptase High Spec Activity-5000 U View
AMV Reverse Transcriptase in Trehalose-500 U View
AMV Reverse Transcriptase in Trehalose-5000 U View
Lyophilized AMV Reverse Transcriptase in Trehalose-750 U View