NASBA Kits & Reagents
Easily amplify and detect RNA with our NASBA kits
Reverse transcriptase PCR (RT-PCR) is currently one of the most commonly used techniques for performing RNA detection, although it suffers from false positives due to cross-contamination whilst being a time consuming process. A more convenient technique for RNA detection is Nucleic Acid Sequence Based Amplification (NASBA), which is a one-step isothermal process for amplifying RNA. NASBA has demonstrated success in the detection of viral (including SARS-CoV-2) and bacterial RNA from clinical samples.
Each NASBA reaction contains avian myeloblastosis virus-reverse transcriptase (AMV-RT), T7 RNA polymerase and RNase H with reaction buffer. Due to NASBA being an isothermal process, denaturation of dsDNA does not occur and ssRNA can be amplified. Therefore, false negatives for the NASBA reaction are prevented that would otherwise be caused by genomic dsDNA in other techniques like RT-PCR.
We offer a range of NASBA kits for RNA detection, which are designed as single product solutions for any NASBA related research and development. The components of the kit are available either in liquid form or lyophilized form to allow shipping and storage at standard room temperature.
We also provide AMV-RT as a standalone product for purchase, available in glycerol storage buffer in addition to Trehalose storage buffer (suitable for lyophilization):
|AMV Reverse Transcriptase||1000 U||View|
|AMV Reverse Transcriptase||25000 U||View|
|AMV Reverse Transcriptase||-||5000 U||View|
|AMV Reverse Transcriptase in Trehalose||-||1,000 U||View|
|AMV Reverse Transcriptase in Trehalose||-||5,000 U||View|
|AMV-RT High Spec Activity||-||5,000 U||View|
|AMV-RT High Spec Activity||-||500 U||View|