
NASBA Kits & Reagents
Easily amplify and detect RNA with our NASBA kits
Reverse transcriptase PCR (RT-PCR) is currently one of the most commonly used techniques for performing RNA detection, although it suffers from false positives due to cross-contamination whilst being a time consuming process. A more convenient technique for RNA detection is Nucleic Acid Sequence Based Amplification (NASBA), which is a one-step isothermal process for amplifying RNA. NASBA has demonstrated success in the detection of viral (including SARS-CoV-2) and bacterial RNA from clinical samples.
Each NASBA reaction contains avian myeloblastosis virus-reverse transcriptase (AMV-RT), T7 RNA polymerase and RNase H with reaction buffer. Due to NASBA being an isothermal process, denaturation of dsDNA does not occur and ssRNA can be amplified. Therefore, false negatives for the NASBA reaction are prevented that would otherwise be caused by genomic dsDNA in other techniques like RT-PCR.
Benefits
- An improvement upon RT-PCR due to higher amplification kinetics
- More cost-effective to perform as the reaction occurs isothermally at 41°C and won’t require costly thermocycling equipment
- Coronaviruses (including SARS-CoV-2) are being detected and quantified by only RNA genome amplification and not proviral DNA copies
- Possible to be used for the detection of human mRNA sequences without risking DNA contamination
- Due to it only being a three-enzyme process, amplification of nucleic acid sequences more than 109 copies can be performed in <90 minutes
NASBA Kits
We offer a range of NASBA kits for RNA detection, which are designed as single product solutions for any NASBA related research and development. The components of the kit are available either in liquid form or lyophilized form to allow shipping and storage at standard room temperature.
Name | Packsize | Order |
---|---|---|
3X NASBA Reaction Buffer for NEC-1-24 | 24 reactions | View |
6X Nucleotide Mix for NEC-1-24 | 24 reactions | View |
Diluent for LEM-10 | 65uL | View |
Lyophilized Reaction Buffer | 10 reactions | View |
NASBA Enzyme Cocktail (wet mix) | 24 reactions | View |
NASBA Lyophilized Kit (10 reactions) | 10 reactions | View |
NASBA Wet Kit | 24 reactions | View |
AMV-RT
We also provide AMV-RT as a standalone product for purchase, available in glycerol storage buffer in addition to Trehalose storage buffer (suitable for lyophilization):
Name | Packsize | Order |
---|---|---|
10X Reaction Buffer for AMV RT | 1.0 mL per 1.000 U of RT | View |
AMV Reverse Transcriptase in Trehalose | 1.000 U | View |
AMV Reverse Transcriptase in Trehalose | 5.000 U | View |
AMV-RT High Spec Activity | 5.000 U | View |
AMV-RT High Spec Activity | 500 U | View |
AMV-RT High Spec Activity | Bulk | View |
AMV-RT High Spec Activity Bulk | Bulk | View |