RNArchive

Ambient storage and protection of RNA samples for decades

Stabilize RNA in solid or liquid form at room temperature

• Protection for years at room temperature

• Safe and secure shipping with no dry ice

• Undergo up to 5 rehydration cycles with no loss in RNA integrity

 

Simple workflow

High levels of RNA protection come with a simple process. Just collect, dry and rehydrate your samples when required with molecular biology grade water. Your samples with be able to withstand 5 rehydration cycles.

Consistently high RIN scores after long-term storage

Shipping RNA on dry ice has become increasingly common. This has lead to a huge rise in expense, but also an increase in the loss and damage of samples on transit. Improper dry ice maintenance causes the thawing and degradation of samples. AMSBIO offers a convenient solution to eliminate dry ice, expensive packaging and overnight shipping.

 

As well as reducing costs RNArchive will provide peace of mind as the risk of sample thawing is totally negated. The robust protection at room temperature ensure the highest quality and safety of your RNA samples, allowing highly efficient subsequent research.

FastDryers

FastDryers are a simple and convenient way to dry
biosamples following application to RNArchive (96-
well microplates, racks of 0.3 ml cluster tubes, and 0.5 ml
screw-cap tubes) and RNArchive (0.5 ml screw-cap tubes
and 0.3 ml cluster tubes),

Application VolumeFast Dryer Drying TimeBiosafety Hood Drying Time
20µL10 hours~18 hours
21-50µL16 hours~24 hours

RNArchive Frequently Asked Questions

What are the two options for stabilizing RNA in RNArchive?

Option 1 - Following application to RNArchive use RNA in liquid form for up to 100 hrs at room temperature
(21-25°C) or on ice. RNArchive conveys additional stability to RNA in liquid form by inactivating trace RNase, simplifying sample handling. Following the 100 hour period, dry the sample down, or store RNA according to your typical protocol.

Option 2 - Following application to RNArchive dry RNA and store or transport at ambient temperature. Following rehydration, RNArchive conveys additional stability to RNA in liquid form for up to 8 hours at room temperature (21-25°C) or on ice. Following the 8 hour post-recovery period, dry your sample down again for storage or store
according to your typical protocol.

Can RNA be rehydrated and dried multiple times?

Yes, RNA in RNArchive can be rehydrated and re-dried up to five times.

Is it safe to keep RNA at room temperature (21-25°C) during the 16 hour drying process?

Yes, the RNArchive matrix protects RNA in the liquid state at room temperature during the drying process. This protection extends for up to 48 hours.

What is the composition of the storage solution after recovery?

After addition of molecular-grade water, your samples will be in the same buffer they were stored in at the time of application.

How should I store my recovered RNA?

Following recovery, RNA may be stored for up to 8 hours at room temperature (21-25°C), and then dry down or store according to your typical protocol.

Can I use the recovered RNA directly for downstream applications?

Additional purification is not required prior to performing downstream applications. Similar RNA quality is maintained before and after recovery.

What is RNArchive? Is RNArchive composed of a filter, beads or paper?

RNArchive is not a filter, beads, silica column or paper. RNArchive is a water soluble, chemical matrix

When removing DNA contamination from my RNA preparation does the inhibitor in RNArchive interfere with digestion of gDNA using DNase I.?

Yes, the RNase inhibitor in RNArchive is an inhibitor of DNase I. However, because of the low concentration of inhibitor it should be easy to swamp the inhibition by simply increasing the amount of DNase I used.

Can RNArchive tubes be used to store DNA?

No, the chemical matrix used to store DNA is not the same as the chemical matrix used to store RNA.

Can I apply an RNA sample that is smaller than the recommended 20 µl?

Yes, but special care and handling is recommended. When applying the sample be especially careful to place it in the very bottom of the tube. When recovering the sample we recommend using a minimum of twice the original volume to ensure you rehydrate all the sample. Refer to our tutorial on handling small sample volumes for further details.

Does the use of RNArchive interfere with library construction?

At its normal concentration range there is no effect on the polymerase reaction and we have done many library preps on RNArchive protected samples with no problems at all. Furthermore, as the sample moves thought the library construction the RNArchive is quickly diluted away.

Will RNArchive interfere with the removal of RNA template after the first strand reaction?

Yes, unless the RNArchive is diluted by >10-100 times compared to its normal concentration it will be a potent inhibitor of the RNase used to remove the starting RNA.