RNAssure

Room temperature stabilization and protection for purified RNA

AMSBIO's RNAssure stops RNA degradation with patented Active Chemical Protection™ (ACP).

• Hassle-free integration with your chosen purification method

• Handle RNA for 3 days without freezing

• Maintain the integrity of your samples for downstream processes

• Use with both phenol and column-based methods

RNAssure is compatible with all major workflows

RNAssure Elution Tubes replace those of standard purification kits to efficiently collect purified RNA as it is eluted from your chosen purification column.

RNA stabilised by RNAssure does not require additional clean up steps to be utilised downstream applications like quantitation, gel or Bioanalyzer™ analysis, RT-PCR, and NGS library preparation. The patented Active Chemical Protection™ technology works to inactivate all nucleases, protect against oxidation and prevent adherence of RNA to plastic surfaces.

Storage ConditionLength of Protection
Room temperature (15-25°C)3 days
Refrigeration (2-8°C)2 weeks
Frozen (<-70°C)>1 year

RNAssure Frequently Asked Questions

What is RNAssure? Is RNAssure composed of a filter, beads, or paper?

RNAssure is a water soluble, chemical matrix provided dried in a green microcentrifuge fliptop tube. RNAssure is not composed of any filters, beads, or paper.

Can I use the RNA in RNAssure directly for downstream applications?

Yes, additional purification is not required prior to performing downstream applications. The
RNAssure does not interfere with any downstream protocol.

When removing DNA contamination from my RNA preparation does the inhibitor in RNAssure interfere with digestion of gDNA using DNase I?

No, the inhibitor concentration used in RNAssure will not interfere with common DNase treatment protocol.

Can I elute an RNA sample in a volume that is smaller than the recommended 20 μL?

Collecting less than 20 μL will increase the concentration of the active ingredient which may inhibit downstream analysis.

Can I use RNAssure nonstandard RNA purification kits?

Yes, RNAssure Elution Tube can be used without the need for manufacturer kits with spin columns. After the final elution or resuspension step, collect 20 – 150 μL of the RNA sample and add it to the RNAssure Elution tube. Mix by gently tapping for 10-15 seconds or pipetting up and down 10 times to solubilize and mix the RNAssure stabilization agent into the purified RNA sample

Can I use RNAssure with whole blood stabilization tubes (PAXgene, Tempus, etc.)?

Yes, RNAssure Elution Tubes are compatible with whole blood stabilization tubes.

Does the use of RNAssure interfere with library construction?

No, at its normal concentration there is no effect on the reverse transcriptase or polymerase reactions.

Will RNAssure interfere with the removal of RNA template after the first strand reaction?

RNAssure contains potent inhibitors of all RNases. To ensure proper removal of RNA templates, ensure that the input RNAssure stabilized RNA sample constitutes less than 10% of the total reaction volume.

Is there a minimum or maximum concentration of RNA that can be used?

There is no limitation of RNA concentration. However, follow the RNA extraction kit manufacturer’s recommendation and do not exceed the recommended total RNA amount

Will RNAssure work with small RNA molecules (e.g., miRNAs, tracrRNA, etc.)?

Yes, RNAssure is a chemical matrix that effectively protects all forms of RNA.

Will the presence of RNAssure change my 260/280 or 260/230 ratio

RNAssure will not affect 260/280 ratio. However, it has an absorbance of approximately 0.4 OD at 230 nm (e.g. NanoDrop analysis) and will cause a slightly higher 230 nm sample reading and therefore reduce the 260/230 ratio value. If desired, a “blank” or control sample can be made. To do this, add same volume of nuclease-free water to an empty RNAssure tube.