The ABLE 30 mL Disposable Bioreactor
Benefits of Spheroid Culture
The ABLE 30 mL Disposable Bioreactor provides a combination of efficiency, embryoid body formation, cell differentiation, and cost savings. The Bioreactor comes ready-to-use for non-adherent cell growth, eliminating the need for high-priced, high-performance extracellular matrix (ECM) proteins for coating plasticware. One bioreactor enables expansion for up to 5 × 10⁷ cells; approximately equivalent to cell yield from ten 10 cm culture dishes or ten 6-well plates. Ten dishes have approximately 550 cm² of combined surface area.
Single cell seeding provides for exponential growth
Human iPS cells (1231A3) were maintained on iMatrix–511–coated plates and grown in StemFit® medium. Cells were harvested and dissociated into single cells using TrypLE Select (ThermoFisher), washed and counted. The single-cells were then seeded at 10⁵ cells/mL in StemFit medium supplemented with 10 μM Y27632 and transferred to the ABLE 30 mL Disposable Bioreactor with constant spinner agitation at 55 rpm. Cells were harvested on day 2 and day 4, and spheroids dispersed by TrypLE Select, stained with trypan blue, and counted.
ABLE Bioreactor Sizes
ABLE System Configuration
The ABLE 3D System has only two components: a six-channel magnetic stirrer platform (30 mL size shown), and an electrical controller and motor unit. The Stirrer Platform is placed inside a CO₂ cell culture incubator allowing the temperature, humidity and gas controls to be passively managed. The controller unit attaches magnetically to the outside of the incubator, and the motor is connected to the Stirrer Platform by a cable.
Controller and motor
Product Information Table
|ABLE 100 mL Disposable Bioreactor (4 units)||4 units||View|
|ABLE 30mL Disposable Bioreactor (6 units)||6 units||View|
|ABLE 500 mL Disposable Bioreactor (1 units)||1 units||View|
|ABLE 5mL Disposable Bioreactor (6 units)||6 units||View|
|ABLE Bioreactor Magnetic Stir System Base 30 mL and 100 mL (1 unit)||1 each||View|
|ABLE Bioreactor Magnetic Stir System Base 5 mL (1 unit)||1 each||View|
|ABLE Bioreactor Magnetic Stir System Controller and Motor||1 each||View|
- Matsuura K. Fabrication of mouse embryonic stem cell-derived layered cardiac cell sheets using a bioreactor culture system. PloS One 7: e52176 (2012).
- Matsuura K. Creation of human cardiac cell sheets using pluripotent stem cells. Biochem Biophy Res Commun 425: 321-327 (2012).