StemFit® For Differentiation
The next generation supplement for differentiation of hPSCs
StemFit® For Differentiation (“StemFit® Diff”) is a chemically defined and human/animal component-free supplement for differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells to multiple lineages. It can be used with a variety of different induction factors or cytokines to support differentiation along ectoderm, mesoderm or endoderm lineages. StemFit® Diff can be combined with StemFit® Basic media, using iMatrix-511 laminin as extracellular matrix, to support clinical research applications of human pluripotent stem cell (hPSC)-derived cells/tissues by providing defined and xeno-free culture systems for both expansion and differentiation. StemFit® Diff is provided as 5X concentrate and is intended to be used with basal medium (DMEM, DMEM/F12, RPMI1640, etc. and appropriate induction factors or cytokines.
Features
- Supplement for differentiation of human ES and iPS cells to multiple lineages
- Chemically defined composition minimizes lot-to-lot variation and enables consistent cell differentiation
- Animal-origin free composition minimizes risk of immunogenic contamination
Applications
- Spontaneous differentiation of human iPSCs via embryoid body (EB) formation
- Directed differentiation of iPSCs into endoderm, mesoderm and ectoderm lineages
Real World Application
Scientists at the University of Tokyo and Yokohama City University have developed an animal-derived product free protocol for mass production of mini-livers, revolutionizing clinical trials and treatment for liver diseases using StemFit for Differentiation.
Spontaneous Differentiation
StemFit® Diff supports spontaneous differentiation of human induced pluripotent stem cells (hiPSCS) via embryoid body (EB) formation. EBs are aggregates of pluripotent stem cells that form the primary germ layers as shown in Fig. 2. We also provide Lipidure®-COAT Plates – ultra-low attachment plates that promote cell aggregation, making them the perfect solution for EB formation.
Other recommended products for EB generation
Lineage-Specific Differentiation
Large populations of hiPSCs can be directly differentiated into each of the three germ layers: endoderm, mesoderm, and ectoderm, using StemFit® Diff. Below are protocols for differentiating iPSCs into each of the germ layers. Please note that there are some cases where protocol optimization would be required.
Endoderm Generation from iPSCs
The endoderm is the innermost germ layer, which gives rise to many internal organs, including the pancreas and liver. The protocol below shows the differentiation of iPSCs (1231A3 on iMatrix-511) into mature hepatocytes (MH).
Other recommended products for endoderm generation
Mesoderm Generation from iPSCs
The mesoderm is the middle germ layer, which gives rise to muscle (cardiac, skeletal, and smooth muscle in the gut), the kidneys, and red blood cells. The protocol below shows the differentiation of iPSCs (1231A3 on iMatrix-511) into paraxial mesoderm (PM) and septum transversum mesenchyme (STM).
Other recommended products for mesoderm generation
Ectoderm Generation from iPSCs
The ectoderm is the outermost germ layer, which gives rise to skin cells of the epidermis, neuron cells, and pigment cells. The protocol below shows the differentiation of iPSCS (1231A3 on iMatrix-511 into dopaminergic neurons (DAN).
Featured Citations
A human iPS cell myogenic differentiation system permitting high-throughput drug screening.
Uchimura, T. et al. (2017). Stem Cell Research. 25:98–106.
Human iPS cell-derived dopaminergic neurons function in a primate Parkinson’s disease model.
Kikuchi T, et al. (2017). Nature ;548(7669):592–6.
Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.
Tohyama, S. et al. (2017). Stem Cell Reports; 9(5):1406–14.
Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates.
Kawamura, T. et al. (2016). Stem Cell Reports ;6(3):312–20.
Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.
Sugai, K. et al. (2016). Molecular Brain. 19;9(1).
Derivation of Mesenchymal Stromal Cells from Pluripotent Stem Cells through a Neural Crest Lineage using Small Molecule Compounds with Defined Media.
Fukuta, M. et al.(2014).PLOS ONE. 2;9(12): e112291–1
