iMatrix Recombinant Laminin E8 Fragments

All the iMatrix series in box

1 vial x iMatrix-111,  1 vial x iMatrix-221, 1 vial x iMatrix-332, 1 vial x iMatrix-411, 1 vial x iMatrix-511, and 1 vial x iMatrix-511 silk

- For preparing suitable substrates for each cell type in microphysiological systems (MPS)

- For replicating the in vivo environment of cells in cell culture

- For exploring substrates for the differentiation of pluripotent stem cells into targeted cells

- For exploring substrates for primary cell culture

For maintenance and expansion of Pluripotent Stem Cells

A highly purified and refined product of human recombinant laminin-511 E8 fragment expressed by CHO-S cells. iMatrix-511 easily enables extended culture of human pluripotent stem cells and has swiftly established itself as the gold standard for stem cell culture.

The clinical grade version of iMatrix-511 is also available; iMatrix-511 MG meets the Standards of Biological Ingredients set by the PMDA, a Japanese government agency homologous to the FDA. To enquire about iMatrix™-511 MG, please email us at [email protected].

For maintenance and expansion of Pluripotent Stem Cells

iMatrix-511 Silk is human recombinant Laminin-511 E8 fragment with the same functions and performance as those of iMatrix-511, the only difference being is that it is expressed in transgenic silkworm cocoon rather than CHO-S cells. The highly productive transgenic silkworms make recombinant laminin fragment at a competitive price.

For maintenance and expansion of Pluripotent Stem Cells with GMP compatibility

iMatrix-511MG is for the maintenance and expansion of iPSCs meeting regulatory requirements for clinical applications. It is a highly purified and refined product of human recombinant laminin-511 E8 fragment expressed from the equivalent CHO-S cells expression system as the RUO iMatrix-511.

Our iMatrix™ products perfectly fit your workflow as you can easily move from basic research using our RUO iMatrix-511 to clinical use with iMatrix-511MG. With our clinical grade products you have the same versatility and benefits of our RUO products such as using the Pre-Mix Method saving you both time and money and the Scraper-Free Method increasing detachment efficiency and cell viability.

For induction of vascular endothelial cells from hPSCs

Laminin-411 (α4β1γ1) is the major isoform that lines the basement membrane of blood vessels. It is thought to be involved in maintaining vascular homeostasis by binding to the integrin α6β1 protein on the cell surface of vascular endothelial cells. In addition, it is also known to adhere to leukocytes and platelets, important for the immune system.

iMatrix-411 contains the essential laminin-411 E8 fragments which retains full integrin binding activity but lacks binding activity to other cell matrix components. iMatrix-411 can be used to efficiently induce vascular endothelial cells and bile duct epithelial cells from hPSCs/iPSCs by binding to integrin α6β1.

For enrichment and maintenance of cardiomyocytes

Laminin 221 (α2β2γ1) is abundantly present in the basement membrane of muscle tissues such as cardiac muscle and skeletal muscle. It binds to the integrin α7X2β1 protein, which is selectively expressed in muscle tissue. It is thought to be involved in the differentiation and functional maintenance of muscle cells.

iMatrix-221 is recombinant laminin-221 E8 fragment and exhibits high adhesive activity and selectivity as a culture medium for cardiomyocytes and skeletal muscle cells.

Induction of differentiation of vascular endothelial cells from ES/iPS cells

Laminin-332 (α3β3γ2) is present in keratinocytes and cornea and is known to bind to integrin α3β1 and α6β4. It is also essential for epithelial cell adhesion to the basement membrane.

iMatrix-332 is the highly purified E8 region (including the integrin binding site) of human laminin 332.

Induction of differentiation from human iPS cells to hepatoblast-like cells

Laminin-111 (α1β1γ1) is a major substrate for nerve axon growth, both in vivo and in vitro. It's also the main component of the industry standard substrates used in cell culture: Matrigel and Cultrex BME. Laminin-111 is known to be present in the liver and bind to integrin α7X2β1 and α6β1 proteins, and is thought to be involved in the maintenance of liver tissue function.

Our iMatrix-111 is the highly purified E8 region (including the integrin binding site) of human laminin-111 and has been used for the differentiation of iPSCs into hepatocyte-like cells.