The store will not work correctly when cookies are disabled.
Menu
  •  
  • Call:+44 (0) 1235 828 200
  • orContact Us
  • Call:+44 (0) 1235 828 200
  • orContact Us
  • Call:+41 (0) 91 604 55 22
  • orContact Us
  • Call:+49 (0) 69 779099
  • orContact Us
  • Call:+31 (0) 72 8080244
  • orContact Us
  • Call:+1 (617) 945 5033
  • orContact Us
AMSBIO_RGB_Colour_logoAMSBIO_RGB_Colour_logoAMSBIO_RGB_Colour_logoAMSBIO_RGB_Colour_logo
  • My Account
    • log in
    • create account
  • My Cart
    My Cart1
Menu
  • Products
    • Close
    • Antibodies
      • Close
        • Monoclonal
          • Close
          • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
        • Polyclonal
          • Close
          • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
        • Secondary Antibodies
          • Close
          • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
    • Biorepository
      • Close
        • Biofluids
          • Close
          • Image
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • DNA
          • Close
          • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • FFPE Cell Line Controls
          • Close
          • Image
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Primary Cells
          • Close
          • Image
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Proteins
          • Close
          • Image
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • RNA
          • Close
          • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Tissues
          • Close
          • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • FDA Tissue Arrays –
    • Bioseparation & Electrophoresis
      • Close
        • Bioseparation Reagents
          • Close
          • Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Chromatography Media
          • Close
          • Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Electrophoresis Equipment
          • Close
          • Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Magnetic Beads
          • Close
          • Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Pre-cast Gels
          • Close
          • Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
    • Cells & Cell Culture
      • Close
        • 3D Cell Culture & Extracellular Matrices
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Cell Culture Kits
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Cell Lines
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Cell Line Lysates
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • FFPE Cell Line Controls
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Media and Reagents
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Primary Cells
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Stem Cells
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Transfection Reagents
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Viral Delivery
          • Close
          • Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
    • Kits & Assays
      • Close
        • Apoptosis Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Cell Culture Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Oris™ Cell Migration Assays –
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Oris™ Cell Invasion Assays –
        • Cell Proliferation and Viability Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Conjugation Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • ELISA Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Enzyme Activity Assays
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Exosome Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Metabolism Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Other Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Protein Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Stem Cell Kits
          • Close
          • Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
    • Molecular Biology
      • Close
        • cDNA Clones
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Cloning Vectors
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Genome Editing
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Magnetic Beads
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Molecular Biology Enzymes
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Nucleic Acid Isolation Kits
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Other
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • PCR Reagents
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • RNAi
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Transfection Reagents
          • Close
          • Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
    • Proteins & Peptides
      • Close
        • Cell Line Lysates
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Magnetic Beads for Protein Applications
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Mass Spec Standards
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Overexpression FFPE Cell Line Sections
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Overexpression Lysates
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Peptides and Blocking Peptides
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Purified Natural Proteins
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Recombinant Proteins
          • Close
          • Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Synthetic Nanodiscs –
    • Reagents & Labware
      • Close
        • Carbohydrates
          • Close
          • Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Gold & Silver Coated Substrates
          • Close
          • Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Labware and Equipment
          • Close
          • Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Other Enzymes
          • Close
          • Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Reagents & Consumables
          • Close
          • Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
  • Research Areas
    • Close
    • 3D Cell Culture
      • Close
      • Imaging Solution –
      • Collagen Scaffold and Bio-Ink
      • ECM Hydrogels –
      • Cryopreservation of Organoids –
    • Cancer Research
    • Cardiovascular
    • Cell & Gene Therapy
      • Close
      • Genetically engineered iPSCs –
    • Cellular Metabolism
      • Close
      • Cell lines for diabetes and obesity research –
      • Cellular Respiration –
      • Ion Permeation Pathways –
      • Metabolic Biomarkers –
      • Oxidative Stress: Antioxidants and Free Radicals –
      • Post Glycolytic Pathways –
      • Post Krebs Cycle Pathways –
    • Cultured Meat
    • Epigenetics
    • Exosomes
    • Glycobiology
    • Immunology
    • Inflammation, Arthritis and Allergy
    • Neuroscience
    • Stem Cells
      • Close
      • Source
      • Grow and Store
      • Differentiate
        • Close
        • iPSC Differentiation Kits –
        • iPSC-Derived Cells –
        • Skeletal Muscle Differentiation Kits –
        • StemFit® For Differentiation Media –
        • StemFit Purotein® Recombinant Proteins –
      • Manufacture
      • Quality Control of Stem Cells –
  • Resources
  • Custom Services
    • Close
    • Lentivirus & AAV & more
      • Close
        • AAV –
        • Adenovirus –
        • Herpes Simplex Virus (HSV) –
        • Lentivirus packaging services –
        • MMLV Retrovirus –
        • MSCV Retrovirus –
        • Vaccinia Virus (VACV) –
        • Vesicular Stomatitis Virus (VSV) –
    • Molecular Biology
      • Close
        • Aptamer Design and Synthesis
        • CRISPR Gene Editing
        • Cytometry Service
        • Exosome Research Services
        • In Vitro Synthesised mRNA Service
        • NanoString Service
        • RNA & DNA Synthetic Standards
        • Spatial Biology Service –
        • Stable Cell Lines
        • Vector Cloning Service
    • Peptide & Antibody
      • Close
        • Licensing –
        • Monoclonal Antibody Service
        • Peptide Synthesis
        • Phospho Polyclonal Antibody
        • Polyclonal Antibody Service
        • Recombinant Monoclonal Antibody Service
    • Protein Expression
      • Close
        • Bacterial Protein Expression
        • Baculovirus Protein Expression
        • Cell Free Protein Expression
        • Mammalian Protein Expression
    • CAR-T Development –
    • Cell Based Assays
    • Drug Screening Service
    • Histochemistry
    • Magnetic Beads
    • Stem Cell Differentiation
    • Tissue Procurement
    • Custom Services Request Form –
  • GMP –
    • Close
    • AMSBIO’s Guide to GMP
    • GMP-Compliant Products & Services –
  • Support
    • Close
    • Contact Us
    • Distributors
    • FAQ –
    • Ordering Information
    • Product Citations
  • About Us
    • Close
    • Amsbio Blog
    • Careers
    • Collaborate With Us –
    • Europa Biosite –
    • Events
Account
Settings
Language
USD Store
  • GBP Store
  • EUR Store
  • CHF Store
  • Secondary USD Store
  • Home
  • News
  • Using Lentivectors to Label Organoids

Lipidure: Generate Cell Repellent Surfaces in Microfluidic Devices
January 19, 2022
Modelling SARS-CoV-2 infection using iPSCs and organoids
June 18, 2021

Using Lentivectors to Label Organoids

AMSBIO have supplied custom lentivectors to Francesco Pampaloni and his team (Lotta Hof, Till Moreth, Michael Koch) at the Buchmann Institute for Molecular Life Sciences (BMLS) in Frankfurt, Germany allowing them to visualize cell nuclei and F-actin cytoskeleton in their organoids, as published in BMC Biology.

Organoids are morphologically heterogeneous 3D cell culture systems (differentiated in vitro from stem cells or progenitor cells) that provide an ideal model for understanding the principles of collective cell behavior in organs during development, homeostasis, regeneration, and pathogenesis.

Michael Koch, has commented:

As epithelial organoids have a stem cell-like character, which makes them difficult to transduce, they require optimised transduction protocols. AMSBIO custom high-titre lentiviral particles offered us an ideal solution to this issue. By labelling epithelial organoids with H2B-eGFP and LifeAct-mCherry using AMSBIO lentivectors/lentiviral particles, we were able to study dynamic cellular processes in organoid development (by light sheet microscopy/LSFM) over several days with a stable fluorescence signal.

These custom lentivectors enabled us to gain intricate insights into highly dynamic processes in epithelial organoid development like formation, polarisation, and luminal expansion. Throughout our research, AMSBIO offered great support through discussing possible options in vector design for optimal delivery and expression of our organoids. We highly recommend AMSBIO custom lentivirus for fluorescently labelling demanding/delicate cells in long-term live microscopy studies and would not hesitate to use their service again.

Using Lentivectors to Label Organoids

AMSBIO’s lentiviral system is a novel gene delivery tool using lentivectors for gene expression or knockdown. Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro. Unlike traditional retroviral system, the AMSBIO lentivirus is much more actively imported into the nuclei of non-dividing cells and stably integrated into the host cell’s genome independent of cell cycle. Offering full customization, including choice of promoter, marker, tags, number of ORFs expressed and titer and amount of lentivirus, AMSBIO can meet all your custom lentivirus needs.

Dr Maja Petkovic – a business unit manager at AMSBIO commented “We were delighted to be selected by the BMLS researchers to supply lentivirus expressing fluorescent fusion proteins H2B-eGFP to visualise cell nuclei and LifeAct-mCherry to visualise the F-actin cytoskeleton thereby enabling them to track the cellular dynamics of their organoid development”. She added “Our lentiviral vectors are valuable gene delivery tools. We can offer a custom service to make ready-to-use constitutive or inducible over-expression lentivirus for any specific gene. We will sub-clone your selected genes into our lentivirus expression vectors and produce expression lentivirus for each gene”.

Order Lentiviral Particles

  • Ready-to-use Lentiviral Particles
  • Custom Design
  • Other Viral Delivery Systems

Published Citations

Hof, L., Moreth, T., Koch, M., Liebisch, T., Kurtz, M., Tarnick, J., … & Pampaloni, F. (2021). Long-term live imaging and multiscale analysis identify heterogeneity and core principles of epithelial organoid morphogenesis. BMC biology, 19(1), 1-22.

Related Product Info Pages

Lentivirus & AAV & more

Lentiviruses for SARS-CoV-2 & COVID-19 Research

Related Resources

We couldn't find any records.

Need Help Finding a Product?

Our reagent specialists are to help you find the best product for your application. Please Call or Email us and we will be happy to help you find the right product for the job.

Call us at

+41 (0) 91 604 55 22

+49 (0) 69 779099

+44 (0) 1235 828 200

+31 (0) 72 8080244

+1 (617) 945 5033

Email Us

Subscribe to our newsletter

Be the first to hear about upcoming events, product news, special offers, application notes, and more

  • This field is for validation purposes and should be left unchanged.

Products

  • Biorepository
  • Molecular Biology
  • Antibodies
  • Proteins & Peptides
  • Cells & Cell Culture
  • Reagents & Labware
  • Kits & Assays
  • Bioseparation & Electrophoresis

Custom Services

  • Protein Expression
  • Lentivirus & Adenovirus
    & AAV
  • Peptide & Antibody
  • Tissue Procurement
  • Histochemistry
  • Stem Cell Differentiation
  • Cell Based Assays
  • Drug Screening Service
  • CAR-T Development
  • Magnetic Beads
  • Molecular Biology

Support

  • Product Citations
  • Contact Us
  • FAQ
  • Ordering Information
  • Distributors

About Us

  • Partners
  • Events
  • News
  • Careers

AMSBIO is a leading provider of quality life science research reagents and services.

Reg. in England and Wales no. 02117791.
ISO 9001 registered firm. Ⓒ2024

EORI Number: GB502237295000

Website Terms of Use - Terms of Sale - Privacy Policy Modern Slavery Statement