Using Lentivectors to Label Organoids

June 29, 2021

Developing a Cell Therapy for Parkinson’s Disease

June 17, 2021

Modelling SARS-CoV-2 infection using iPSCs and organoids

In an exciting new study, Dr Kazuo Takayama (CiRA, Kyoto University) has successfully modelled the SARS-CoV-2 life cycle in undifferentiated iPSCs, which were previously uninfectable. This ground breaking research has shown how iPSCs and organoids can be used in COVID-19 drug discovery and to investigate differences in SARS-CoV-2 infection caused by variations in genetic background.

Dr Takayama gave a unique insight into his research at the ISSCR Virtual Annual Meeting 2021. You can find the on demand recording of the talk further down this page.

This talk was presented by AMSBIO in partnership with the manufacturers of StemFit® cell culture media, iMatrix™ recombinant laminin ECM and STEM-CELLBANKER® cryopreservation solutions, as used in this research.

Dr Takayama commented: “Since we used eight ACE2-iPS cell lines in this study, we used a very large volume of StemFit medium and iMatrix ECM. Although many cells are required for COVID-19 drug evaluation, iPS cells cultured with StemFit and iMatrix have a high replication capacity, so that sufficient cell numbers can easily be prepared – and can easily be cryopreserved as required using STEM-CELLBANKER.

Rabbit Polyclonal Anti-SARS-CoV-2 Antibody used in Fig 2 is available from AMSBIO.

Speaker Profile: Dr. Kazuo Takayama is a junior associate professor (PI) at Center for iPS cell Research and Application (CiRA), Kyoto University. He started his laboratory at CiRA in March 2020, coinciding with the COVID-19 pandemic. He is developing differentiation technology of airway organoids for COVID-19 research. He is also using iPS cell panels to recapitulate individual differences in the symptoms of COVID-19. In this seminar, he will introduce the latest findings and challenges of COVID-19 research utilizing organoids and iPS cells.

Further reading of interest:

1. Suzuki, T., Ito, Y., Sakai, Y., Saito, A., Okuzaki, D., Motooka, D., … & Takayama, K. (2020). Generation of human bronchial organoids for SARS-CoV-2 research. BioRxiv. Citing STEM-CELLBANKER for GMP cryopreservation of Human Bronchial Organoids

2. Sano, E., Deguchi, S., Sakamoto, A., Mimura, N., Hirabayashi, A., Muramoto, Y., … & Takayama, K. (2021). Modeling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells. Iscience, 24(5), 102428. Citing StemFit cell culture medium and iMatrix-511 as ECM

3. Serrano-Aroca, Á., Takayama, K., Tuñón-Molina, A., Seyran, M., Hassan, S. S., Choudhury, P. P., … & Brufsky, A. M. Carbon-Based Nanomaterials: Promising Antiviral Agents to Combat COVID-19 in the Microbial Resistant Era.

4. Takayama, K. (2020). In vitro and animal models for SARS-CoV-2 research. Trends in pharmacological sciences, 41(8), 513-517.

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