For effective organoid culture
RSPO1 is the critical ingredient used in the maintenance and proliferation of mouse and human organoid progenitor stem cells. To date, positive results have been reported with stem cells of gastric, colonic, intestinal, pancreatic and liver lineage by thought leaders in the industry such as Dr. Hans Clevers (Hubrecht Institute) and Dr. Calvin Kuo (Stanford University). These cells can be passaged indefinitely and can produce unlimited amount of RSPO1 protein, allowing laboratories conducting research with organoids to save time and money.
Roof plate-specific Spondin-1 (R-Spondin 1 or RSPO1), also known as CRISTIN3, is a 27 kDa secreted activator protein that belongs to the R-Spondin family. R-Spondins positively regulate Wnt/beta-catenin signalling, most likely by acting as a ligand for LGR4-6 receptors and an inhibitor for ZNRF3. R-Spondin-1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal.
The 293T cell line is stably transfected to express murine Rspo1 with an N-terminal HA epitope tag and fused to a C-terminal murine IgG2a Fc fragment. This cell line is used to produce either purified Rspo1 or Rspo1 conditioned media. The murine Rspo1 protein has been used extensively in organoid culture to maintain Lgr5+ stem cells, and the FC and HA tags make it easy to purify or characterize.
- Cell line expresses recombinant mouse RSPO1 protein
- Positively regulates Wnt/β-catenin signalling
- Essential medium component for most organoid culture models
- Purified protein and conditioned medium from our cell line has been used for culturing both human and mouse organoids.
A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression.
Fumagalli, A., Suijkerbuijk, S. J., Begthel, H., Beerling, E., Oost, K. C., Snippert, H. J., ... & Drost, J. (2018). Nature Protocols, 13(2), 235.
Salivary gland stem cells age prematurely in primary Sjögren's syndrome.
Pringle, S., Wang, X., Verstappen, G. M., Terpstra, J. H., Zhang, C. K., He, A., ... & Vissink, A. (2019). Arthritis & Rheumatology, 71(1), 133-142.
Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche,
Ootani, A., et al. (2009_ , Nat Med, 15(6): p.701-706
Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro.
Barker, N., et al. (2010). Cell Stem Cell, 6(1): p.25-36
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., et al., (2009). Nature, 459(7244): p.262-265
Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
Sato, T. and H. Clevers, (2013) . Science, 340(6137): p.1190-1194
Isolation and in vitro expansion of human colonic stem cells
Jung, P., et al, (2011). Nat Med, 17(10): p.1225-7
*Featured Page Image - Diﬀerentiation of Organoids into Hepatocytes on ECM from AMSBIO using RSPO1 from same cell line as from AMSBIO. Image courtesy of Dr. Meritxell Huch (MPI-CBG, Dresden, Germany)