Heparanase and Hyaluronidase Activity Kits

Effectively measure enzyme activity

AMSBIO supplies Razie assay kits to measure enzyme activity of heparanase and hyaluronidase in cell culture supernatants, human plasma, biological fluids and tissue samples. Our kits have been used for research into anti-inflammatory responses, kidney disease and cancer research. Heparanase and hyaluranidase enzymes both play an important role in the extracellular matrix (ECM). Our kits provide a solution to the performance and standardization problems found with traditional measurement techniques used for these types of enzymes.

Features

  • Suitable for inhibitor screening
  • Non-radioactive
  • Fast and easy to use
  • Sensitive and specific
  • Use universal 96-well plate format ideal for inhibitor studies

Glycosaminoglycan Substrates

Citations

Expression and activity of hyaluronidases HYAL-1, HYAL-2 and HYAL-3 in the human intervertebral disc.
Krupkova, O.,Greutert, H., Boos, N et al (2019) European Spine Journal, 1-11
(Note that our Chondroitinase ABC has also been to model intervertebral disc degeneration).

The Effects of Gold and Silver Nanoparticles on an Enzymatic Reaction
Between Horseradish Peroxidase and 3,3',5,5'-Tetramethylbenzidine
Morris, B & Behzad, F., (2014) Biochemistry & Pharmacology: Open access, 3(6):

Heparanase Is Essential for the Development of Acute Experimental Glomerulonephritis.
Garsen, M., et al (2016) The American Journal of Pathology 186(4): 805-815.

Endothelin-1 induces proteinuria by heparanase-mediated disruption of the glomerular glycocalyx.
Garsen, M., et al (2016). Journal of the American Society of Nephrology, 27(12), 3545-3551.

Vitamin D attenuates proteinuria by inhibition of heparanase expression in the podocyte
Garsen, M., et al (2015) The Journal of Pathology 237(4): 472-481.

Regulation of heparanase by albumin and advanced glycation end products in proximal tubular cells
Masola, V., et al (2011) Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1813(8): 1475-1482