Heparanase and Hyaluronidase Activity Kits
Effectively measure enzyme activity
AMSBIO supplies Razie assay kits to measure enzyme activity of heparanase and hyaluronidase in cell culture supernatants, human plasma, biological fluids and tissue samples. Our kits have been used for research into anti-inflammatory responses, kidney disease and cancer research. Heparanase and hyaluranidase enzymes both play an important role in the extracellular matrix (ECM). Our kits provide a solution to the performance and standardization problems found with traditional measurement techniques used for these types of enzymes.
- Suitable for inhibitor screening
- Fast and easy to use
- Sensitive and specific
- Use universal 96-well plate format ideal for inhibitor studies
Expression and activity of hyaluronidases HYAL-1, HYAL-2 and HYAL-3 in the human intervertebral disc.
Krupkova, O.,Greutert, H., Boos, N et al (2019) European Spine Journal, 1-11
(Note that our Chondroitinase ABC has also been to model intervertebral disc degeneration).
The Effects of Gold and Silver Nanoparticles on an Enzymatic Reaction Between Horseradish Peroxidase and 3, 3', 5, 5'-Tetramethylbenzidine.
Morris, B., & Behzad, F. (2014) Biochemistry & Pharmacology: Open Access, 2014.
The Effects of Gold and Silver Nanoparticles on an Enzymatic Reaction
Between Horseradish Peroxidase and 3,3',5,5'-Tetramethylbenzidine
Morris, B & Behzad, F., (2014) Biochemistry & Pharmacology: Open access, 3(6):
The β-D-endoglucuronidase heparanase is a danger molecule that drives systemic inflammation and correlates with clinical course after open and endovascular thoracoabdominal aortic aneurysm repair: Lessons learnt from mice and men.
Martin, L., Gombert, A., Chen, J., Liebens, J., Kalder, J., Marx, G., ... & Schuerholz, T. (2017). Frontiers in Immunology, 8, 681.
This paper cites Ra001-BE-K (Heparanase Activity Assay Kit with Positive Control Enzyme), alongside AMS.E03H0100 (ELISA to measure quantity of Mouse Heparanase) and AMS.E0623Ge (General ELISA Kit for Heparan sulfate)
The Synthetic Antimicrobial Peptide 19-2.5 Interacts with Heparanase and Heparan Sulfate in Murine and Human Sepsis
Martin, L., et al (2015) PloS one 10(11): e0143583.
Heparanase Is Essential for the Development of Acute Experimental Glomerulonephritis.
Garsen, M., et al (2016) The American Journal of Pathology 186(4): 805-815.
Endothelin-1 induces proteinuria by heparanase-mediated disruption of the glomerular glycocalyx.
Garsen, M., et al (2016). Journal of the American Society of Nephrology, 27(12), 3545-3551.
Vitamin D attenuates proteinuria by inhibition of heparanase expression in the podocyte
Garsen, M., et al (2015) The Journal of Pathology 237(4): 472-481.
Heparanase activity in alveolar and embryonal rhabdomyosarcoma: implications for tumor invasion
Masola, V., et al (2009) BMC Cancer 9(1), 1
Heparanase and syndecan-1 interplay orchestrates FGF-2-induced epithelial-mesenchymal transition in renal tubular cells
Masola, V., et al (2011) Journal of Biological Chemistry, jbc-M111
Regulation of heparanase by albumin and advanced glycation end products in proximal tubular cells
Masola, V., et al (2011) Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1813(8): 1475-1482