Chondroitinase ABC

Enzyme & Antibodies for Neuroscience and Glycoanalysis

Chondroitin Sulfate Proteoglycans (CSPGs) are involved in the inhibition of axon regeneration after various forms of damage to the Central Nervous System, including stroke and spinal cord injury. The enzyme Chondroitinase ABC (purified from Proteus vulgaris) degrades these CSPGs, and has been shown to promote functional recovery and neural regeneration in addition to its role as a tool in glycoanalysis.

Chondroitin Sulfate “stub” antibodies

Along with our Chondroitinase ABC, we also supply three monoclonal antibodies that recognise unsulfated (0S)4-sulfated (4S) & 6-sulfated (6S) Chondroitin & Dermatan Sulfate, following Chondroitinase ABC digestion of proteoglycans or perineuronal nets.

Citations

Functional regeneration of intraspinal connections in a new in vitro model.
Heidemann, M., et al (2014) Neuroscience 262: 40-52.

Therapeutic efficacy of microtube-embedded chondroitinase ABC in a canine clinical model of spinal cord injury
Hu, H.Z., et al (2018) Brain 141(4): 1017-1027.
See also Moon and Bradbury (doi: 10.1093/brain/awy067) for a scientific commentary on this research.

Cortical processing in awake, behaving rats after removal of perineuronal nets.
Lensjo, K.K., et al (2013) Poster exhibited at SfN Neuroscience 2013 Conference.

 A large animal model that recapitulates the spectrum of human intervertebral disc degeneration.
Gullbrand, S. E., Malhotra, N. R., Schaer, T. P., Zawacki, Z., Martin, J. T., Bendigo, J. R., ... & Mauck, R. L. (2017) Osteoarthritis and cartilage25(1), 146-156.

Circulating glycosaminoglycan species in septic shock.
Nelson, A., et al (2014) Acta Anaesthesiologica Scandinavica 58(1): 36-43.

Heparanase activates the syndecan-syntenin-ALIX exosome pathway.
Roucourt, B., et al (2015) Cell Research 25: 412-428.

Chondroitinase Gene Therapy for Spinal Cord Injury.
Hu, J., Curinga, G. M., & Smith, G. M. (2015).  Extracellular Matrix, 139-149 (Clone 3B3)

AAV vector-mediated secretion of chondroitinase provides a sensitive tracer for axonal arborisations.
Alves, J. N., Muir, E. M., Andrews, M. R., Ward, A., Michelmore, N., Dasgupta, D., ... & Rogers, J. H. (2014) Journal of neuroscience methods, 227, 107- (Clone 2B6, also biotin-HABP)

Mapping Proteoglycan Functions with Glycosidases.
Cortes, M., Cortes, L. K., & Schwartz, N. B. (2015) Glycosaminoglycans: Chemistry and Biology, 443-455. (Clones 1B5, 2B6, 3B3 & 10E4)

 Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis.
Developmental Dynamics, 242(6), 778-789.(Clones 1B5, 2B6, 3B3 & 3G10)

Anatomically shaped tissue-engineered cartilage with tunable and inducible anticytokine delivery for biological joint resurfacing.
Moutos, F. T., Glass, K. A., Compton, S. A., Ross, A. K., Gersbach, C. A., Guilak, F., & Estes, B. T. (2016) Proceedings of the National Academy of Sciences, 201601639. (Clone 2B6)

Chondroitinase improves anatomical and functional outcomes after primate spinal cord injury.
Rosenzweig, E. S., Salegio, E. A., Liang, J. J., Weber, J. L., Weinholtz, C. A., Brock, J. H., ... & Tuszynski, M. H. (2019). Nature neuroscience22(8), 1269. (clone 2B6)

Combined chondroitinase and KLF7 expression reduce net retraction of sensory and CST axons from sites of spinal injury.
Wang, Z., Winsor, K., Nienhaus, C., Hess, E., & Blackmore, M. G. (2017) Neurobiologyof disease, 99, 24-35. (Clone 2B6: Also cites our Chondroitinase ABC and Proteoglycan Detection Kit cat.# 280560-N)

Background/Reviews

Review: Manipulating the extracellular matrix and its role in brain and spinal cord plasticity and repair.
Burnside, E.R. & Bradbury, E.J. (2014) Neuropathology and applied neurobiology, 40(1): 26-59.

Fell‐Muir Lecture: Chondroitin sulphate glycosaminoglycans: fun for some and confusion for others.
Caterson, B. (2012) International journal of experimental pathology 93(1): 1-10.

Freeing the brain from the perineuronal net
Fox, K. & Caterson, B. (2002) Science, 298(5596), 1187-1189.