Hyaluronic Acid Binding Protein
Bind and detect HA in Multiple Applications
Hyaluronic Acid or Hyaluronan (HA) is a glycosaminoglycan (GAG) with a simple conserved structure a homogenous non-branching polymer with the same repeating disaccharide structure without sulfation ((GlcAβ(1→3)GlcNAcβ(1→4))). It is widespread in the extracellular matrix, playing many roles in health and disease.
Versican G1-domain as HA Binding Protein
AMSBIO’s rHABP was launched as a replacement for the HABP formerly available from Seikagaku. It is produced as a recombinant protein in E. coli BL21(DE3)RIL transfected with human versican G1-domain expression vector pRK172VG1 and is available in purified and biotinylated formats.
rHABP specifically detects Hyaluronic Acid (HA), binding to HA from all species and tissues. It does not cross-react to other Glycosaminoglycans or to DNA.
Recombinant production means that our rHABP has higher safety compared with animal-derived HABP.
See Clark et al (2011) details on expression and comparison with Seikagaku's biotin-HABP from bovine cartilage, carried out by Tony Day and colleagues at Manchester. This paper also cites a number of our antibodies to specific glycosaminoglycans: 10E4 (pan Heparan Sulfate), 1B5, 2B6, 3B3 (Chondroitin Sulfate & Dermatan Sulfate 'stubs’), LY111 (native Chondroitin-4-Sulfate) and 5D4 (pan Keratan Sulfate).
The biotin-HABP product supplied by AMSBIO was vital for us to be able to visualise the tissue distribution of hyaluronan in mouse vs. naked mole-rat skin. The images were so brilliant that we have started using biotin-HABP in other studies, e.g. looking at the effects of colitis on colonic hyaluronan distribution.
The material properties of naked mole-rat hyaluronan.
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Perineuronal net formation during the critical period for neuronal maturation in the hypothalamic arcuate nucleus.
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Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain.
Godin, A. G., Varela, J. A., Gao, Z., Danné, N., Dupuis, J. P., Lounis, B., ... & Cognet, L. (2017). Nature Nanotechnology 12, 238–243.
Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1.
Johnson, L. A., Banerji, S., Lawrance, W., Gileadi, U., Prota, G., Holder, K. A., ... & Jackson, D. G. (2017). Nature Immunology.
AAV vector-mediated secretion of chondroitinase provides a sensitive tracer for axonal arborisations.
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Genome-Wide Association Study of Golden Retrievers Identifies Germ-Line Risk Factors Predisposing to Mast Cell Tumours.
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Molecular composition and expression pattern of the extracellular matrix in a mossy fiber-generating precerebellar nucleus of rat, the prepositus hypoglossi.
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Hyaluronan accumulates with high-fat feeding and contributes to insulin resistance.
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Early Genetic Restoration of Lubricin Expression in Trangenic Mice Mitigates Chondrocyte Peroxynitrite Release and Caspase-3 Activation.
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The oncolytic adenovirus VCN-01 as therapeutic approach against pediatric osteosarcoma.
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Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat.
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Molecular weight specific impact of soluble and immobilized hyaluronan on CD44 expressing melanoma cells in 3D collagen matrices.
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