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Hyaluronic Acid Binding Protein

Bind and detect HA in Multiple Applications

Hyaluronic Acid or Hyaluronan (HA) is a glycosaminoglycan (GAG) with a simple conserved structure a homogenous non-branching polymer with the same repeating disaccharide structure without sulfation ((GlcAβ(1→3)GlcNAcβ(1→4))). It is widespread in the extracellular matrix, playing many roles in health and disease.

Versican G1-domain as HA Binding Protein

AMSBIO’s rHABP was launched as a replacement for the HABP formerly available from Seikagaku. It is produced as a recombinant protein in E. coli BL21(DE3)RIL transfected with human versican G1-domain expression vector pRK172VG1 and is available in purified and biotinylated formats.

rHABP specifically detects Hyaluronic Acid (HA), binding to HA from all species and tissues. It does not cross-react to other Glycosaminoglycans or to DNA.

Recombinant production means that our rHABP has higher safety compared with animal-derived HABP.

See Clark et al (2011) details on expression and comparison with Seikagaku's biotin-HABP from ‎bovine cartilage, carried out by Tony Day and colleagues at Manchester. This paper also cites a number of our antibodies to specific glycosaminoglycans: 10E4 (pan Heparan Sulfate)1B5, ‎2B6, 3B3 (Chondroitin Sulfate & Dermatan Sulfate 'stubs’)LY111 (native Chondroitin-4-Sulfate) and 5D4 (pan Keratan Sulfate).

The biotin-HABP product supplied by AMSBIO was vital for us to be able to visualise the tissue distribution of hyaluronan in mouse vs. naked mole-rat skin. The images were so brilliant that we have started using biotin-HABP in other studies, e.g. looking at the effects of colitis on colonic hyaluronan distribution.

Ewan St John Smith, Department of Pharmacology, University of Cambridge (UK)

Citations

The material properties of naked mole-rat hyaluronan.
Kulaberoglu, Y., Bhushan, B., Hadi, F., Chakrabarti, S., Khaled, W., Rankin, K., ... & Frankel, D. (2019). Scientific Reports 9, Article number: 6632.

 Perineuronal net formation during the critical period for neuronal maturation in the hypothalamic arcuate nucleus.
Mirzadeh, Z., Alonge, K. M., Cabrales, E., Herranz-Pérez, V., Scarlett, J. M., Brown, J. M., ... & Zeltser, L. M. (2019). Nature Metabolism, 1(2), 212.

Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain. 
Godin, A. G., Varela, J. A., Gao, Z., Danné, N., Dupuis, J. P., Lounis, B., ... & Cognet, L. (2017). Nature Nanotechnology 12, 238–243.

Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1. 
Johnson, L. A., Banerji, S., Lawrance, W., Gileadi, U., Prota, G., Holder, K. A., ... & Jackson, D. G. (2017).  Nature Immunology.

 AAV vector-mediated secretion of chondroitinase provides a sensitive tracer for axonal arborisations.
Alves, J. N., Muir, E. M., Andrews, M. R., Ward, A., Michelmore, N., Dasgupta, D., ... & Rogers, J. H. (2014) Journal of neuroscience methods, 227, 107-120.

 Genome-Wide Association Study of Golden Retrievers Identifies Germ-Line Risk Factors Predisposing to Mast Cell Tumours. 
Arendt, M. L., Melin, M., Tonomura, N., Koltookian, M., Courtay-Cahen, C., Flindall, N., ... & Murphy, S. (2015). PLoS Genet, 11(11), e1005647.

Molecular composition and expression pattern of the extracellular matrix in a mossy fiber-generating precerebellar nucleus of rat, the prepositus hypoglossi.
Gaál, B., Kecskes, S., Matesz, C., Birinyi, A., Hunyadi, A., & Rácz, É. (2015) Neuroscience letters, 594, 122-126.

Hyaluronan accumulates with high-fat feeding and contributes to insulin resistance. 
Kang, L., Lantier, L., Kennedy, A., Bonner, J. S., Mayes, W. H., Bracy, D. P., ... & Wasserman, D. H. (2013). Diabetes, 62(6), 1888-1896

Early Genetic Restoration of Lubricin Expression in Trangenic Mice Mitigates Chondrocyte Peroxynitrite Release and Caspase-3 Activation. 
Larson, K. M., Zhang, L., Badger, G. J., & Jay, G. D. (2017). Osteoarthritis and Cartilage.

The oncolytic adenovirus VCN-01 as therapeutic approach against pediatric osteosarcoma.
Martínez-Vélez, N., Xipell, E., Vera, B., Zalacain, M., Marrodan, L., Gonzalez-Huarriz, M., ... & Alonso, M. M. (2015).  Clinical Cancer Research, clincanres-1899.

Heterogeneous expression of extracellular matrix molecules in the red nucleus of the rat.
Rácz, É., Gaál, B., & Matesz, C. (2016).  Neuroscience , 322, 1-17.

Molecular weight specific impact of soluble and immobilized hyaluronan on CD44 expressing melanoma cells in 3D collagen matrices. 
Sapudom, J., Ullm, F., Martin, S., Kalbitzer, L., Naab, J., Möller, S., ... & Pompe, T. (2017). Acta Biomaterialia.