Animal & Endotoxin-Free Growth Factors & Cytokines

• Animal free stem cell growth factors and cytokines
• Endotoxin free recombinant proteins
• Bacteria, animal, and human cell free
• Produced in barley plants grown in a high-tech greenhouse with a negative CO₂ footprint

• Endotoxin-free growth factors increase precision and reproducibility of stem cell cultures
• Increased reproducibility leads to more consistency in stem cell experiments
• Increased reproducibility leads to smoother transfer of an already established stem cell culture protocol from pre-clinical research to clinical trials

Endotoxins are an unavoidable by-product in E.coli recombinant protein production and, due to their stability and affinity to proteins, difficult to remove completely. Currently endotoxin levels in recombinant protein products below 1 EU/mg (<0.1 ng/mg) are accepted as a safe limit, however there has long been evidence to suggest the level of endotoxin that will not result in an adverse response varies between cell types, with unwanted effects observed below this accepted limit.

In an embryoid bodies-based in vitro stem cell study, endotoxin exposure, as low as 0.005 ng/ml, was demonstrated to seriously effect stem cell differentiation by inhibiting mesoderm formation. This concentration correlates to adding growth factors with 0.1 ng/µg (~1 EU/µg) bacterial endotoxins to a stem cell culture.