Animal & Endotoxin-Free Growth Factors & Cytokines

Increase the reproducibility of your stem cell research

The success of stem cell research depends on the development of optimal cell culture systems. Both expansion and differentiation of stem cells are heavily reliant on growth factor and cytokine supplements, with the activity and purity of these recombinants being critical to successful research and clinical applications. Despite recognized limitations, E. coli and mammalian cells remain the dominant expression systems for commercial recombinant protein production. Although significant improvements in manufacture have been made, both systems present a risk of unwanted contamination from pathogens and endotoxins.

AMSBIO now offers recombinant growth factors and cytokines produced in a plant-based expression system that utilizes barley seed to cut the risks associated with endotoxin, viral infection and other human pathogen infection.

Why use endotoxin-free growth factors?

  • Endotoxin-free growth factors increase precision and reproducibility of stem cell cultures
  • Increased reproducibility leads to more consistency in stem cell experiments
  • Increased reproducibility leads to smoother transfer of an already established stem cell culture protocol from pre-clinical research to clinical trials

Endotoxins are an unavoidable by-product in E.coli recombinant protein production and, due to their stability and affinity to proteins, difficult to remove completely. Currently endotoxin levels in recombinant protein products below 1 EU/mg (<0.1 ng/mg) are accepted as a safe limit, however there has long been evidence to suggest the level of endotoxin that will not result in an adverse response varies between cell types, with unwanted effects observed below this accepted limit.

In an embryoid bodies-based in vitro stem cell study, endotoxin exposure, as low as 0.005 ng/ml, was demonstrated to seriously effect stem cell differentiation by inhibiting mesoderm formation. This concentration correlates to adding growth factors with 0.1 ng/µg (~1 EU/µg) bacterial endotoxins to a stem cell culture.

NameDatasheetPacksize Order
Epithelial Growth Factor Human - EGF - Animal Free100 ug View
Recombinant Human Interleukin-2 (IL-2) - Animal Free10 ug View
Recombinant Human Interleukin-2 (IL-2) - Animal Free100 ug View
Recombinant Human Interleukin-2 (IL-2) - Animal Free1000 ug View
Recombinant Human Interleukin-2 (IL-2) - Animal Free50 ug View
Recombinant Human Interleukin-3 (IL-3) - Animal Free10 ug View
Recombinant Human Interleukin-3 (IL-3) - Animal Free100 ug View
Recombinant Human Interleukin-3 (IL-3) - Animal Free50 ug View
Recombinant Human Interleukin-4 (IL-4) - Animal Free10 ug View
Recombinant Human Interleukin-4 (IL-4) - Animal Free100 ug View
Recombinant Human Interleukin-4 (IL-4) - Animal Free1000 ug View
Recombinant Human Interleukin-4 (IL-4) - Animal Free50 ug View
Recombinant human BMP-2 - Animal Free10 ug View
Recombinant human BMP-2 - Animal Free-100 ug View
Recombinant human BMP-2 - Animal Free-1000 ug View
Recombinant human Flt-3 ligand - Animal Free10 ug View
Recombinant human Flt-3 ligand - Animal Free100 ug View
Recombinant human Flt-3 ligand - Animal Free1000 ug View
Recombinant human Flt-3 ligand - Animal Free50 ug View
Recombinant human glial cell line derived neurotrophic factor (GDNF) - Animal Free10 ug View
Recombinant human glial cell line derived neurotrophic factor (GDNF) - Animal Free100 ug View
Recombinant human glial cell line derived neurotrophic factor (GDNF) - Animal Free1000 ug View
Recombinant human glial cell line derived neurotrophic factor (GDNF) - Animal Free50 ug View
Recombinant human granulocyte colony-stimulating factor (G-CSF) - Animal Free10 ug View
Recombinant human granulocyte colony-stimulating factor (G-CSF) - Animal Free100 ug View
Recombinant human granulocyte colony-stimulating factor (G-CSF) - Animal Free1000 ug View
Recombinant human granulocyte colony-stimulating factor (G-CSF) - Animal Free50 ug View
Recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF) - Animal Free10 ug View
Recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF) - Animal Free100 ug View
Recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF) - Animal Free1000 ug View
Recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF) - Animal Free50 ug View
Recombinant human keratinocyte growth factor (KGF) - Animal Free10 ug View
Recombinant human keratinocyte growth factor (KGF) - Animal Free100 ug View
Recombinant human keratinocyte growth factor (KGF) - Animal Free1000 ug View
Recombinant human keratinocyte growth factor (KGF) - Animal Free50 ug View
Recombinant human leukemia inhibitory factor (hLIF) - Animal Free10 ug View
Recombinant human leukemia inhibitory factor (hLIF) - Animal Free100 ug View
Recombinant human leukemia inhibitory factor (hLIF) - Animal Free1000 ug View
Recombinant human leukemia inhibitory factor (hLIF) - Animal Free50 ug View
Recombinant human macrophage colony stimulating factor (M-CSF) - Animal Free10 ug View
Recombinant human macrophage colony stimulating factor (M-CSF) - Animal Free100 ug View
Recombinant human macrophage colony stimulating factor (M-CSF) - Animal Free50 ug View
Recombinant human stem cell factor (SCF) - Animal Free10 ug View
Recombinant human stem cell factor (SCF) - Animal Free100 ug View
Recombinant human stem cell factor (SCF) - Animal Free1000 ug View
Recombinant human stem cell factor (SCF) - Animal Free50 ug View
Recombinant human tumour necrosis factor, alpha (TNF-alpha) - Animal Free10 ug View
Recombinant human tumour necrosis factor, alpha (TNF-alpha) - Animal Free100 ug View
Recombinant human tumour necrosis factor, alpha (TNF-alpha) - Animal Free1000 ug View
Recombinant human tumour necrosis factor, alpha (TNF-alpha) - Animal Free50 ug View
Recombinant human vascular endothelial growth factor 165 (VEGF) - Animal Free10 ug View
Recombinant human vascular endothelial growth factor 165 (VEGF) - Animal Free100 ug View
Recombinant human vascular endothelial growth factor 165 (VEGF) - Animal Free1000 ug View
Recombinant human vascular endothelial growth factor 165 (VEGF) - Animal Free50 ug View
Recombinant mouse leukemia inhibitory factor mLIF / LIF - Animal Free10 ug View
Recombinant mouse leukemia inhibitory factor mLIF / LIF - Animal Free100 ug View
Recombinant mouse leukemia inhibitory factor mLIF / LIF - Animal Free1000 ug View
Recombinant mouse leukemia inhibitory factor mLIF / LIF - Animal Free50 ug View
Recombinant mouse stem cell factor (SCF) - Animal Free10 ug View
Recombinant mouse stem cell factor (SCF) - Animal Free100 ug View
Recombinant mouse stem cell factor (SCF) - Animal Free1000 ug View
Recombinant mouse stem cell factor (SCF) - Animal Free50 ug View