CAR-T Cell Research

Cells, recombinant proteins, and assays for CAR-T research

Personalized treatment for cancer reached a new milestone with the FDA approval of CAR-T cells for treatment of leukemia and lymphoma. There is now rapid growth in research on the therapeutic uses of CAR-T cells.

At AMSBIO, we strive to provide tools at the cutting edge of cancer research. We produce a growing list of experimental cell lines, recombinant proteins and screening services to advance the search for new CAR-T cell therapies.

How does CAR-T cell therapy work?

A therapeutic CAR (chimeric antigen receptor) is a transmembrane protein designed with an extracellular domain based on an antibody single-chain variable fragment (scFv) and intracellular signaling domains derived from the TCR g chain, along with other costimulatory receptors. The scFv provides a specific binding domain that recognizes target proteins on cancer cells. A patient’s own T cells are isolated and activated, then transfected with a gene expressing the CAR. This reprograms the T cells to identify and attack tumor cells expressing the target protein, creating personalized immune cells designed to specifically target the patient’s cancer.

The first FDA approved treatments were CAR-T cells directed against the B cell protein CD19. Success with CD19 has inspired new research identifying additional targets for several blood cancers and even solid tumors. For example, early clinical trials using CAR-T treatment suggest BCMA and CD22 are promising targets for multiple myeloma and acute lymphoblastic leukemia. CD19-targeting CAR-T cells are also in development as co-therapies with therapeutic antibodies targeting the PD-1:PD-L1 and CD28:CTLA4 pathways. The aim of these early studies is to determine if CAR-T cells can overcome the challenges of adaptive resistance to immune checkpoint therapies.

Cell Lines

Name	Datasheet	Packsize	Order
BCMA / Luciferase - CHO Recombinant Cell Line	-	2 vials	View
BCMA CHO Recombinant Cell Line (High Expression)	-	2 vials	View
BCMA CHO Recombinant Cell Line (Low Expression)	-	2 vials	View
CD123 (IL3Ra)-CHO Recombinant Cell Line (High Expression)		2 vials	View
CD123 (IL3Ra)-CHO Recombinant Cell Line (Low Expression)		2 vials	View
CD123 (IL3Ra)-CHO Recombinant Cell Line (Medium Expression)		2 vials	View
CD47 - HEK293 Cell Line	-	2 vials	View
ICOSL (B7-H2) -CHO Recombinant Cell Line	-	2 vials	View
PD-L1 - CHO Recombinant Cell Line		2 vials	View
PMSA (FOLH1) - CHO Recombinant Cell Line (High, Medium, or Low Expression)		2 vials	View
PMSA (FOLH1) - CHO Recombinant Cell Line (Low Expression)	-	2 vials	View
PMSA (FOLH1) - CHO Recombinant Cell Line (Medium Expression)		2 vials	View

Assays & Proteins

Name	Packsize	Order
BCMA, Fc-Fusion, Avi-Tag HiPâ„¢	100 ug	View
BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit	96 reactions	View
CD19, Fc-Fusion (IgG1), Avi-Tag	100 ug	View
CD22, Fc-fusion, Avi-Tag HiPâ„¢	100 ug	View
CD38, His-Tag (Human), HiPâ„¢	100 ug	View
CD38, His-Tag (Mouse), HiPâ„¢	100 ug	View
CD38, His-Tag, APC-labeled	100 ug	View
CD47 (Monkey), Fc Fusion (Human), Avi-Tag HiPâ„¢	100 ug	View
CD47, Fc fusion	100 ug	View
CD47, Fc fusion, Avi-Tag (Human) HiPâ„¢	100 ug	View
CD47, Fc-Fusion, Streptavidin-Labeled (Human) HiPâ„¢	100 ug	View
CD47, His-tag	100 ug	View
ROR1, Fc-Fusion (IgG1), Avi-Tag	10 ug	View
ROR1, Fc-Fusion (IgG1), Avi-Tag, Biotin-Labeled	10 ug	View
ROR1, GST-tag	10 ug	View

CAR-T Cells

Name	Datasheet	Packsize	Order
'Mock' ScFv Control	-	1 x 10^6 cells/vial	View
3x Beam 2-TM28-CD28-CD3 zeta (Mock)	-	1 x 10^6 cells/vial	View
B7H4-TM28-CD28-CD3	-	1 x 10^6 cells/vial	View
BCMA-2-TM-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
BCMA-4-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
BCMA-4-TM8-4-1BB-CD3 zeta		1 x 10^6 cells/vial	View
CD123-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD133 scFv-CD28-CD3?	-	1 x 10^6 cells/vial	View
CD138-Beam 1-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD19-Beam 2-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD19-CD22-4-1BB-CD3	-	1 x 10^6 cells/vial	View
CD19-CD22-4-1BB-CD3-T2A-RQR8 (Ritiximab suicide switch)	-	1 x 10^6 cells/vial	View
CD19-CD22-4-1BB-CD3-T2A-tEGFR (Cetuximab sucicide switch)		1 x 10^6 cells/vial	View
CD19-TM-28-GITR-CD3 zeta	-	1 x 10^6 cells/vial	View
CD19-TM-8-GITR-CD3 zeta	-	1 x 10^6 cells/vial	View
CD19scFv-4-1BB-CD3?		1 x 10^6 cells/vial	View
CD19scFv-CD28	-	1 x 10^6 cells/vial	View
CD19scFv-CD28-4-1BB		1 x 10^6 cells/vial	View
CD19scFv-CD28-4-1BB-CD3		1 x 10^6 cells/vial	View
CD19scFv-CD28-CD3?		1 x 10^6 cells/vial	View
CD19scFv-FLAG-CD28-CD3?		1 x 10^6 cells/vial	View
CD20-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD22-Beam 2-TM28-Cd28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD22-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD22-TM8-4-1BB-CD3 zeta	-	1 x 10^6 cells/vial	View
CD24-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD33-Beam 2-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD33-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD37-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD37-TM8-4-1BB-CD3 zeta	-	1 x 10^6 cells/vial	View
CD38-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD4-Beam 1-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CD47-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
CS1p-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
EGFR-TM28-CD3zeta-GITR		1 x 10^6 cells/vial	View
EGFR-TM28-GITR-CD3z		1 x 10^6 cells/vial	View
EGFRscFv-4-1BB-CD3?		1 x 10^6 cells/vial	View
EGFRscFv-CD28-CD3?		1 x 10^6 cells/vial	View
EpCAMscFv-CD28-CD3? NheI restriction site introduced, N-terminal of scFV (amino acids: AS)	-	1 x 10^6 cells/vial	View
EpCAM1 scFv-CD28-CD3?		1 x 10^6 cells/vial	View
GPC3 scFv-CD28-CD3?	-	1 x 10^6 cells/vial	View
Her2 scFv-4-1BB-CD3z-GFP		1 x 10^6 cells/vial	View
Her2 scFv-CD28-CD3?		1 x 10^6 cells/vial	View
Human ROR1-TM28-4-1BB-CD3 zeta	-	1 x 10^6 cells/vial	View
Humanized BCMA-2-TM28-CD28-CD3 zeta		1 x 10^6 cells/vial	View
Humanized 1 CD19-Beam 1-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
Humanized 1-CD19-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
Humanized CD47-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
Humanized CEA-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
MCAM-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
Mesothelin scFv-4-1BB-CD3? (2nd)	-	1 x 10^6 cells/vial	View
Mesothelin scFv-CD28-4-1BB-CD3? (3rd)	-	1 x 10^6 cells/vial	View
Mesothelin scFv-CD28-CD3? (2nd)	-	1 x 10^6 cells/vial	View
Mesothelin scFv-FLAG-4-1BB-CD3? (2nd)	-	1 x 10^6 cells/vial	View
Mesothelin scFv-FLAG-CD28-CD3?	-	1 x 10^6 cells/vial	View
Mesothelin-TM28-CD28-4-1BB-CD3 zeta	-	1 x 10^6 cells/vial	View
Mouse ROR-1-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
NGFR-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
PDL-1-TM28-CD28-CD3 zeta	-	1 x 10^6 cells/vial	View
VEGFR2scFv-CD28-CD3?	-	1 x 10^6 cells/vial	View
iCas9 HA-T2A-antiCD19scFv-CD28-CD3??GGGS-FLAG		1 x 10^6 cells/vial	View
iCas9-T2A-antiCD19scFv-CD28-CD3?		1 x 10^6 cells/vial	View
no scFv-CD28-CD3?	-	1 x 10^6 cells/vial	View

Cell Media & Activation Beads

Name	Datasheet	Packsize	Order
CAR-T Cell Expansion Medium (human serum)		500 ml	View
CAR-T Cell Medium (Fetal Bovine Serum)		500 ml	View
CD28/CD3 PM-T-Cell Activation/Expansion Beads	-	10 ml	View
CD28/CD3 PM-T-Cell Activation/Expansion Beads	-	2 ml	View

Non-Transduced Cells

Name	Packsize	Order
Non-transduced Macrophage Cells	1 x 10^6 cells/vial	View
Non-transduced T CD4+ CM cells	1 x 10^6 cells/vial	View
Non-transduced T CD4+ EM cells	1 x 10^6 cells/vial	View
Non-transduced T SCM	1 x 10^6 cells/vial	View
Non-transduced T cells CD4+	1 x 10^6 cells/vial	View
Non-transduced T cells CD8+	1 x 10^6 cells/vial	View
Non-transduced T naïve	1 x 10^6 cells/vial	View
Non-transduced T-cells, ALL	1 x 10^6 cells/vial	View
Non-transduced T-cells, CLL	1 x 10^6 cells/vial	View
Non-transduced T-cells, NHL	1 x 10^6 cells/vial	View
Non-transduced T-cells, healthy donor	1 x 10^6 cells/vial	View

PBMCs

Name	Packsize	Order
Monkey PBMC cells	1 x 10^6 cells/vial	View
PBMC-ALL	1 x 10^6 cells/vial	View
PBMC-CLL	1 x 10^6 cells/vial	View
PBMC-NHL	1 x 10^6 cells/vial	View

Additional Resources