CAR-T Cell Research

Cells, recombinant proteins, and assays for CAR-T research

Personalized treatment for cancer reached a new milestone with the FDA approval of CAR-T cells for treatment of leukemia and lymphoma. There is now rapid growth in research on the therapeutic uses of CAR-T cells.

At AMSBIO, we strive to provide tools at the cutting edge of cancer research. We produce a growing list of experimental cell lines, recombinant proteins and screening services to advance the search for new CAR-T cell therapies.

We offer knockout cell lines for TCR, B2M, and CIITA, enabling the modeling of universal CAR-T cells. We also provide cell lines expressing common CAR targets like BCMA, CD19, CD20, and more, many of which include the luciferase reporter for simple monitoring. We also offer ready-to-use CAR-expressing cells for assay design/optimization and as positive controls in co-culture assays, as well as eGFP and Luciferase-expressing NK-92 cells for CAR-NK research.

Cell Lines

NamePacksize Order
BCMA / Luciferase - CHO Recombinant Cell Line2 vials View
BCMA CHO Recombinant Cell Line (High Expression)2 vials View
BCMA CHO Recombinant Cell Line (Low Expression)2 vials View
CD123 (IL3Ra)-CHO Recombinant Cell Line (High Expression)2 vials View
CD123 (IL3Ra)-CHO Recombinant Cell Line (Low Expression)2 vials View
CD123 (IL3Ra)-CHO Recombinant Cell Line (Medium Expression)2 vials View
CD47 - HEK293 Cell Line2 vials View
ICOSL-CHO Recombinant Cell Line2 vials View
PD-L1 - CHO Recombinant Cell Line2 vials View
PMSA (FOLH1) - CHO Recombinant Cell Line (High/ Medium/ or Low Expression)2 vials View
PMSA (FOLH1) - CHO Recombinant Cell Line (Low Expression)2 vials View
PMSA (FOLH1) - CHO Recombinant Cell Line (Medium Expression)2 vials View

Recombinant proteins can be used for a variety of purposes for CAR-T cell research. We provide biotin and PE-labeled versions of many of the target antigens for CARs, such as BCMA, CD19, CD22, CD38, CD123, and ROR1. These PE-labeled proteins can bind to CAR-T cells and be detected by flow cytometry, in order to verify CAR-T cell antigen recognition.

Assays & Proteins

NamePacksize Order
BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit96 reactions View
CD22, Fc-fusion, Avi-Tag HiP™100 ug View
CD38, His-Tag (Human), HiP™100 ug View
CD38, His-Tag (Mouse), HiP™100 ug View
CD38, His-Tag, APC-labeled100 ug View
CD47 (Monkey), Fc Fusion (Human), Avi-Tag HiP™100 ug View
CD47, Fc fusion100 ug View
CD47, Fc-Fusion, Streptavidin-Labeled (Human) HiP™100 ug View
CD47, His-tag100 ug View
ROR1, Fc-Fusion (IgG1), Avi-Tag, Biotin-Labeled50 ug View
ROR1, GST-tag10 ug View

CAR-T Cells

NameDatasheetPacksize Order
B7H4 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
BCMA scFv-TM-4-1BB-CD3z1 x 10^6 cells/vial View
BCMA scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Beam 2-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD123 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD133 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD138 scFv-Beam 1-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD19 scFv-Beam 2-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD19 scFv-CD22 scFv-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
CD19 scFv-CD22 scFv-TM-4-1BB-CD3z-T2A-RQR8-1 x 10^6 cells/vial View
CD19 scFv-CD22 scFv-TM-4-1BB-CD3z-T2A-tEGFR1 x 10^6 cells/vial View
CD19 scFv-TM-4-1BB-CD3z1 x 10^6 cells/vial View
CD19 scFv-TM-CD28-1 x 10^6 cells/vial View
CD19 scFv-TM-CD28-4-1BB1 x 10^6 cells/vial View
CD19 scFv-TM-CD28-4-1BB-CD3z1 x 10^6 cells/vial View
CD19 scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
CD19 scFv-TM-FLAG-CD28-CD3z1 x 10^6 cells/vial View
CD19 scFv-TM-GITR-CD3z-1 x 10^6 cells/vial View
CD20 scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
CD22 scFv-Beam 2-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD22 scFv-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
CD22 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD24 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD33 scFv-Beam 2-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD33 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD37 scFv-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
CD37 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD38 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD4 scFv-Beam 1-TM-CD28-CD3z-1 x 10^6 cells/vial View
CD47 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
CS1 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
EGFR scFv-TM-4-1BB-CD3z1 x 10^6 cells/vial View
EGFR scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
EGFR scFv-TM-CD3z-GITR1 x 10^6 cells/vial View
EGFR scFv-TM-GITR-CD3z1 x 10^6 cells/vial View
EpCAM scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
GPC3 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Her2 scFv-TM-4-1BB-CD3z (tEGFR)1 x 10^6 cells/vial View
Her2 scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
Humanized BCMA scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
Humanized CD19 scFv-Beam 1-TM-CD28-CD3z-1 x 10^6 cells/vial View
Humanized CD19 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Humanized CD47 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Humanized CEA scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Humanized ROR1 scFv-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
MCAM scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
MSLN scFv-Beam 1-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
MSLN scFv-TM-4-1BB-CD3z-1 x 10^6 cells/vial View
MSLN scFv-TM-4-1BB-CD3z-FLAG-1 x 10^6 cells/vial View
MSLN scFv-TM-CD28-4-1BB-CD3z1 x 10^6 cells/vial View
MSLN scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
Mock-TM-CD28-CD3z-1 x 10^6 cells/vial View
NGFR scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
No scFv-CD28-CD3z-1 x 10^6 cells/vial View
PD-L1 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
ROR1 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
VEGFR2 scFv-TM-CD28-CD3z-1 x 10^6 cells/vial View
iCas9-T2A-CD19 scFv-TM-CD28-CD3z1 x 10^6 cells/vial View
iCas9-T2A-CD19 scFv-TM-CD28-CD3z-FLAG1 x 10^6 cells/vial View

Cell Media & Activation Beads

NameDatasheetPacksize Order
CAR-T Cell Expansion Medium (human serum)500 ml View
CAR-T Cell Medium(Fetal Bovine Serum)500 ml View
CD28/CD3 PM-T-Cell Activation/Expansion Beads10 ml View
CD28/CD3 PM-T-Cell Activation/Expansion Beads2 ml View

Non-Transduced Primary Cells

NamePacksize Order
CD4+ T cells/ Negatively Selected (Human)10 million cells View
CD8+ T cells/ Negatively Selected (Human)10 million cells View
Non-transduced CD4+ Tem cells  1x10^6 cells/vial View
Non-transduced T CD4+ Tcm cells 1x10^6 cells/vial View
Non-transduced T cells CD4+ T cells1x10^6 cells/vial View
Non-transduced T cells CD8+ T cells1x10^6 cells/vial View
Non-transduced T cells, ALL1x10^6 cells/vial View
Non-transduced T cells, CLL1x10^6 cells/vial View
Non-transduced T cells, NHL1x10^6 cells/vial View
Non-transduced T cells, healthy donor1x10^6 cells/vial View
Non-transduced T cells, GFP+1x10^6 cells/vial View
Non-transduced Tcm cells1x10^6 cells/vial View
Non-transduced Tna®ve®cells®1x10^6 cells/vial View
Normal Human Peripheral Blood Mononuclear Cells/ Frozen100 million cells View
Normal Human Peripheral Blood Mononuclear Cells/ Frozen30 million cells View
Untransduced T Cells1 vial View
Untransduced T Cells5 vials View

PBMCs (Peripheral Blood Mononuclear Cells) play a crucial role in CAR-T research. These cells, including T cells and other immune cells, are isolated from the patient's blood and can be engineered to express CAR receptors specific to cancer antigens. PBMCs serve as the starting material for CAR-T cell production, allowing researchers to study their efficacy, functionality, and potential for targeted cancer therapy.

PBMCs

NamePacksize Order
Normal Human Peripheral Blood Mononuclear Cells/ Frozen100 million cells View
Normal Human Peripheral Blood Mononuclear Cells/ Frozen30 million cells View

Antibodies

NamePacksize Order
Anti-BCMA Antibody (Single-Chain Variable Fragment)|His-Tag100 ug View
Anti-BCMA Antibody (Single-Chain Variable Fragment)|His-Tag50 ug View
Anti-BCMA Antibody100 ug View
Anti-BCMA-Anti-CD19-Anti-CD3 Trispecific Molecule100 ug View
Anti-BCMA-Anti-CD19-Anti-CD3 Trispecific Molecule50 ug View
Anti-BCMA-Anti-CD3 Bispecific Molecule100 ug View
Anti-BCMA-Anti-CD3 Bispecific Molecule50 ug View
Anti-CD19-Anti-CD3 Bispecific Molecule100 ug View
Anti-CD19-Anti-CD3 Bispecific Molecule50 ug View
Anti-CD19-Anti-CD3 IgG format Bispecific Antibody100 ug View
Anti-CD19-Anti-CD3 IgG format Bispecific Antibody50 ug View
Anti-CD20-Anti-CD3 Bispecific Antibody100 ug View
Anti-CD20-Anti-CD3 Bispecific Antibody50 ug View
Anti-CD28 Agonist Antibody (Humanized)100 ug View
Anti-CD28 Agonist Antibody (Humanized)50 ug View
Anti-CD28 Agonist Antibody100 ug View
Anti-CD28 Agonist Antibody50 ug View
Anti-CD3 Agonist Antibody100 ug View
Anti-CD3 Agonist Antibody50 ug View
Anti-PSMA-Anti-CD3 IgG format Bispecific Antibody100 ug View
Anti-PSMA-Anti-CD3 IgG format Bispecific Antibody50 ug View

Lentiviruses are well-suited for introducing CAR genes into primary cells, and our replication-incompetent lentiviruses are particularly advantageous due to their VSV-G pseudotyping. This enhances their safety, stability, and versatility across various cell types, especially T cells in culture. Our high-titer CAR lentivirus products allow for efficient transduction and sustained expression in both resting and actively dividing cells over extended periods of time.

Lentiviruses

NamePacksize Order
Anti-BCMA CAR Lentivirus (Clone C11D5.3 ScFv-CD8-4-1BB-CD3?)50 ul View
Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3?)50 ul View
Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3?; SIN Vector)50 ul View
Anti-CD19-ScFv- CD28-CD3zeta(Bsd)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv- CD28-CD3zeta(GFP-Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv- CD28-CD3zeta(Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv- CD28-CD3zeta(RFP-Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv-4-1BB-CD3zeta(GFP-Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv-4-1BB-CD3zeta(Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv-4-1BB-CD3zeta(RFP-Puro)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv-4-1BB-CD3zeta(no marker)1x10^8 IFU/ml x 200ul View
Anti-CD19-ScFv-CD28-CD3zeta(no marker)1x10^8 IFU/ml x 200ul View
Anti-CD19/CD22 Bispecific CAR Lentivirus (Clones FMC63/m971 ScFv-CD8-4-1BB-CD3?)50 ul View
Anti-CD20 CAR Lentivirus (Clone Leu-16 ScFv-CD8-4-1BB-CD3?)50 ul View
Anti-CD22 CAR Lentivirus (Clone m971 ScFv-CD8-4-1BB-CD3?)50 ul View
CAR negative control: 4-1BB-CD3zeta(Puro)1x10^8 IFU/ml x 200ul View
CAR negative control: Anti-CD19-ScFv- CD28 (Puro)1x10^8 IFU/ml x 200ul View
CAR negative control: Anti-CD19-ScFv-4-1BB (Puro)1x10^8 IFU/ml x 200ul View
CAR negative control: CD28-CD3zeta(Puro)1x10^8 IFU/ml x 200ul View