CD4+ T cells, Negatively Selected (Human)
Product Code
AMS.79752
$0.00
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Cryopreserved vial (10 x 10^6 cells) of CD4+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD8+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD4+ T cells via immunomagnetic separation. Before and after CD4+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreAMSBIOtion medium (Stemcell, #07930) at a controlled rate._x000D_,Source Normal human PBMC from Leukapheresis Sample
| SKU | AMS.79752 |
|---|---|
| Size | 10 million cells |
| Species | Human |
| Gene synonyms | T-Cell Surface Glycoprotein CD4, T-Cell Surface Antigen T4/Leu-3, CD4 Molecule |
| Storage | Store cells at -135°C or colder. Thawed cells should be used immediately for downstream applications. Because these are primary cells, we do not recommend maintaining these cells in culture for long periods of time. |
| Shipping Temp | -80°C (dry ice) |
| Supplier Name | BPS Bioscience Inc |