YFP (Topaz) Lentivirus

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Topaz1, a yellow fluorescence protein (YFP), is one of the brightest and thermally stable green fluorescence proteins (GFP). The YFP (Topaz) Reporter Lentivirus contains replication-defective, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an EF1alpha promoter-driven YFP(Topaz) construct (a.a. sequence below), and confer both YFP (Topaz) expression and puromycin resistance to the target cells. YFP (Topaz) expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. Topaz has an excitation wavelength of 514 nm, an emission wavelength of 527 nm, and extinction coefficient of 94,500M-1cm-1.;
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SKU 79989
Size 500 ul x 2
Alternate SKU AMS.79989
Application Optimizing transduction assays Easily track transduction efficiency over time Generation of YFP (Topaz) Reporter stable cell lines
Accession No AUG68261
Gene synonyms 79703, Topaz1, yellow fluorescence protein lentivirus, GFP lentivirus
Storage Lentiviruses are shipped on dry ice. For long term storage, it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Shipping Temp -80C
Supplier Name BPS