Spike (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter)
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The SARS-CoV-2 Spike Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions also contain the enhanced GFP gene driven by a CMV promoter (Figure 1), therefore, the spike-mediated cell entry can be conveniently determined via eGFP activity. The SARS-CoV-2 Spike pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility.
|Application||1. Study the mechanism of viral transduction. 2. Screening for neutralizing antibodies for SARS-CoV-2 Spike and ACE2.|
|Gene synonyms||covid, covid-19, covid19, coronavirus, spike SARS lentivirus, Spike SARS-CoV-2 GFP reporter, Spike reporter, pseudovirus, pseudovirion, 79981-1, 79981-2|
|Storage||Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.|
|Shipping Temp||-80C/dry ice|