Exosome Quantification
Extracellular Vesicle research needs reliable, affordable and optimized tools for quantification and characterization of vesicles in complex biological samples and cellular models. Here at AMSBIO, we offer our ExoMeasure and ExoFluor kits for quantification and characterization of EV markers by ELISA and FACS, respectively.
We have also introduced our new ExoEnolase Activity Kit, which characterizes the source of EVs by determining the enolase activity.
ExoMeasure
Our patented double sandwich ELISA assay for quantitative and qualitative analysis of vesicles.
ExoMeasure is a successful platform for exosome quantification and characterization from small amounts of human biological fluids or cell conditioned media. It enables reliable and precise quantitative measurement and comparison among samples and individual experiments and provides increased sensitivity in detection of EV markers compared to other analytical methods (i.e FACS, WB)
Applications
- Comprehensive exosome profiling
- Exosome capture and quantification from human biofluids and cell culture media
- A non-invasive pre-clinical method for detection and monitoring of pathological conditions such as inflammation, cancer, neurodegeneration, etc
Advantages
- Ready to use
- No initial exosome purification required
- User friendly and suitable for multiple marker analyses
- A sample kit is available (limited to 3 ELISA strips, 24 wells)
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoMeasure for Overall Exosome capture and quantification from Cell media (COLO1) |  | kit | View |
| ExoMeasure for Overall Exosome capture and quantification from human plasma | | kit | View |
| ExoMeasure for Overall Exosome capture and quantification from human serum | | kit | View |
| ExoMeasure for Overall Exosome capture and quantification from human urine | | kit | View |
| ExoMeasure for Tumor-derived Exosome enrichment and quantification from human plasma (COLO1) | | kit | View |
ExoFluor
Our ready-to-use kit for EV FACS analysis. The kit consists of our ExoPure reagent (exosome isolation), 4 µm beads used for the overall capture of pre-isolated exosomes, our lyophilized exosomes (ExoStd) from cell culture supernatants or human biological fluids as positive control.
The characterization of exosomal proteins (membrane-expressed or internal) is subsequently performed using appropriate detection antibodies against exosome-associated antigens.
Applications
- Exosome isolation and exosome marker characterization via FACS.
- Comprehensive exosome profiling.
- We offer ExoFluor kits for staining of EV markers from human biofluids (plasma, urine, serum, saliva) and from cell culture supernatants.
Advantages
- Ready to use.
- No initial exosome purification required.
- ExoStd (Lyophilized Exosome Standards) for positive control included.
- User-friendly and suitable for multiple marker analyses.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoFluor ready to use kit for analysis of exosomes markers from MSC exosomes | | kit | View |
| ExoFluor ready to use kit for analysis of exosomes markers from cell media | | kit | View |
| ExoFluor ready to use kit for analysis of human PLASMA exosomes | | kit | View |
| ExoFluor ready to use kit for analysis of human SERUM exosomes | | kit | View |
| ExoFluor ready to use kit for analysis of human URINE exosomes | | kit | View |
ExoEnolase
Enolase, also known as (2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase), is a key enzyme in glycolysis. Enolase activity is easily detectable in eukaryotic cell-derived EVs and can be used for evaluating functionality and stability of EVs. Increased enolase activity is associated with tumorigenesis and therefore precise measurement is of great interest for EV-based tumor diagnosis.
Applications
- EV characterization by functional properties
- Determine the enolase activity in purified/isolated EVs
- Determine the functionality and stability of EVs from cell lines
- Mechanistic studies of cancer-originating EVs
Advantages
- Ready to use
- Suitable for measuring enolase activity from fresh, frozen or lyophilized cell-derived EVs
- Small volume of starting material: minimum 1x108 particles per reaction.
- Kit contains Lyophilized EVs as positive control.
- Fluorimetric and colorimetric readout.
| Name | Datasheet | Packsize | Order |
|---|---|---|---|
| ExoEnolase | | kit | View |
