Recombinant Nucleosome (E. coli-derived)|Biotin-labeled|His-Tag

Name: Recombinant Nucleosome (E. coli-derived)|Biotin-labeled|His-Tag
Brand: AMS.52048
SKU: AMS.52048
Availability: InStock

Product Code

AMS.52048

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Overview

Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end))|H2B (a.a. 2-126(end))|H3 (a.a. 2-137(end))|and H4 (a.a. 2-103(end))|expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445|NM_003528|NM_003532|and NM_003548|respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa.

Product Specifications

More Information
SKU	AMS.52048
Size	20 ug
Species	Human
Expression Host	E. coli
Tag	His
Tag position	N-terminal
Label/Conjugate	Biotin
Application	Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E
Accession No	NM_033445, NM_003528, NM_003532, NM_003549
Gene id	NM_033445, NM_003528, NM_003532, NM_003549
Format	Aqueous buffer solution;
Storage	Stable at -80C for at least 6 months from date of receipt# when stored as directed
Shipping Temp	-80C (dry ice)
Supplier Name	BPS
Research Areas	Epigenetics

For more details visit our Histone Methylation page >

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Recombinant Nucleosome (E. coli-derived)|Biotin-labeled|His-Tag

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