EZH2(Y641F) TR-FRET Assay Kit
Product Code
AMS.52680
$0.00
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The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps|making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format|with histone H3 peptide substrate|a Tb-labeled antibody against methylated K27 residue of Histone H3|S-adenosylmethionine|methyltransferase assay buffer|TR-FRET detection buffer|dye-labeled acceptor|and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit|only three simple steps on a microtiter plate are required for methyltransferase detection. First|S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next|antibody is added. Finally|dye-labeled acceptor is added followed by fluorescence reading.
SKU | AMS.52680 |
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Size | 384 reactions |
Application | Great for screening small molecular inhibitors for drug discovery and HTS applications. |
Gene synonyms | EZH2 (Y641F), EED, SUZ12, RbAp48, AEBP |
Storage | Stable at least 6 months from date of receipt# when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |
Research Areas | Epigenetics |