EZH2 (A738T) Chemiluminescent Assay Kit

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AMS.52067
$0.00
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The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (A738T) Chemiluminescent Assay Kit comes in a convenient format|with wells precoated with histone H3 peptide substrate|an antibody against methylated K27 residue of Histone H3|the secondary HRP-labeled antibody|S-adenosylmethionine|methyltransferase assay buffer|and purified EZH2 (A738T) complex for 100 enzyme reactions. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit|only three simple steps on a microtiter plate are required for methyltransferase detection. First|S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next|primary antibody is added. Finally|the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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SKU AMS.52067
Size 96 reactions
Application Great for studying enzyme kinetics and HTS applications.
Storage One year from date of receipt when stored as directed.
Shipping Temp -80C (dry ice)
Supplier Name BPS
Research Areas Epigenetics
For more details visit our Histone Methylation page >