EZH2 (A738T) Chemiluminescent Assay Kit
Product Code
AMS.52067
$0.00
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The EZH2 (A738T) Chemiluminescent Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (A738T)/EED/SUZ12/RbAp48/ AEBP) for screening and profiling purposes. The EZH2 (A738T) Chemiluminescent Assay Kit comes in a convenient format|with wells precoated with histone H3 peptide substrate|an antibody against methylated K27 residue of Histone H3|the secondary HRP-labeled antibody|S-adenosylmethionine|methyltransferase assay buffer|and purified EZH2 (A738T) complex for 100 enzyme reactions. The key to the EZH2 (A738T) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit|only three simple steps on a microtiter plate are required for methyltransferase detection. First|S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next|primary antibody is added. Finally|the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
SKU | AMS.52067 |
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Size | 96 reactions |
Application | Great for studying enzyme kinetics and HTS applications. |
Storage | One year from date of receipt when stored as directed. |
Shipping Temp | -80C (dry ice) |
Supplier Name | BPS |
Research Areas | Epigenetics |