Adenoviral Particles

• Ready to use - simply add the virus to your cell line for target expression
• Safe to use - our adenovirus is produced from a vector with E1 and E3 deletions, resulting in replication-incompetent virus
• Efficient delivery - achieve close to 100% expression in actively dividing and non-dividing mammalian cells in culture or in vivo

We offer over 17,000 sequence-verified human ORFs packaged in adenovirus. If you cannot find the ORF you are looking for, we can clone it and package it into adenovirus with our Adenovirus Custom Service.

We supply a collection of ready-to-use adenovirus controls, suitable for being tranduction controls in a large range of cell lines and types. Each expression adenovirus contains a target expressed under an enhanced human cytomegalovirus (suCMV) promoter. Where applicable, a fluorescent marker (GFP or RFP) is bicistronically expressed under the same promoter, mediated by a 2A element which allows expressing two individual targets.

The adenovirus’ titers (Ifu/ml) are calculated from the positive cell counts of the fluorescent/other marker following transduction into HeLa cells. The represented titers are close to the real infectious units, the PFU titers.

We provide adenovirus  in two formats:

• 200 ul in DMEM medium with 10 % FBS (1×109 ifu/ml);
• 50 ul for in vivo application in PBS solution with 5% sucrose (1×1011 ifu/ml)

We provide a series of adenovirus expressing stem cell factors (OCT3/4, SOX2, LIN28) under a CMV promoter, provided either in culture medium (with 10% FBS) or in PBS (with 5% sucrose) for in vivo applications.