Ab Heparan Sulfate, purified (clone F58-10E4), 50ug pack

Product Code
370255-S
$350.00
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RECOGNITION: The antibody (F58-10E4 clone) reacts with the 10E4 epitope, which is present in many types of heparan sulfate. 10E4 epitope includes (an) N-sulfated glucosamine residue(s) that are critical for the reactivity of the F58-10E4 antibody. The reactivity of the antibody with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparatinase I (Heparinase III) from Flavobacterium heparinum (EC 4.2.2.8). The 10E4 antibody does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA.
More Information
SKU 370255-S
Size 0.05 mg
Host species Mouse
isotype IgM
Species reactivity Broad
Storage 2 - 8 deg C
Shipping Temp Gel Packs
Research Areas Glycobiology
For more details visit our Heparan Sulfate Antibodies page >

Metabolic rewiring and autophagy inhibition correct lysosomal storage disease in mucopolysaccharidosis IIIB (2024)

Product Code: 370255-1; also 370255-S
Author List: Melania Scarcella, Gianluca Scerra, Mariangela Ciampa, Marianna Caterino,Michele Costanzo, Laura Rinaldi, Antonio Feliciello, Serenella Anzilotti, Chiara Fiorentino, Maurizio Renna, Margherita Ruoppolo,Luigi Michele Pavone, Massimo D’Agostino, and Valeria De Pasquale
Journal: iScience 27, 108959, March 15, 2024

Identification of kinesin family member (KIF22) homozygous variants in spondyloepimetaphyseal dysplasia with joint laxity, lepdodactylic type and demonstration of proteoglycan biosynthesis impairment (2024)

Product Code: 370255-S or 370255-1; second citation code not specified; might be 280560-N
Author List: Johanne Dubail, Sophie Rondeau, Caroline Michot, Geneviève Baujat, Yline Capri, Julien Thévenon, Maelle Charpie, Zagorka Pejin, Gilles Phan, Céline Huber,Valérie Cormier-Daire
Journal: Journal of Bone and Mineral Research, 2024, 1–11

Glycosaminoglycan-mediated lipoprotein uptake protects cancer cells from ferroptosis (2024)

Product Code: 370255-S
Author List: Calhoon, D., Sang, L., Divya Bezwada, Kim, N., Basu, A., Hsu, S.-C., Pimentel, A., Brooks, B., La, K., Ana Paulina Serrano, Cassidy, D. L., Cai, L., Vanina Toffessi-Tcheuyap, Margulis, V., Cai, F., Brugarolas, J., Weiss, R. J., DeBerardinis, R. J., Kivanc Birsoy, & Garcia-Bermudez, J.
Journal: BioRxiv (Cold Spring Harbor Laboratory)

Glycosaminoglycan analysis: Purification, structural profiling, and gag–protein interactions (2022)

Product Code: 370255-S; 370260-S
Author List: Amrita Basu & Ryan J. Weiss
Journal: Methods in Molecular Biology, 159–176.

Cellular Chondroitin Sulfate and the Mucin-like Domain of Viral Glycoprotein C Promote Diffusion of Herpes Simplex Virus 1 While Heparan Sulfate Restricts Mobility (2022)

Product Code: 370255-S
Author List: Yara Abidine, Lifeng Liu, Oskar Wallén, Edward Trybala, Sigvard Olofsson, Tomas Bergström and Marta Bally
Journal: Viruses, 14(8)

GRASP depletion‐mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion (2022)

Product Code: 370255-1, 370255-S
Author List: Erpan Ahat1 · Yuefan Song2 · Ke Xia2 · Whitney Reid1 · Jie Li1 · Sarah Bui1 · Fuming Zhang2 · Robert J. Linhardt2 · Yanzhuang Wang1,3
Journal: Cellular and Molecular Life Sciences

A stabilized cxcl9(74–103)-derived peptide selectively inhibits proliferation, adhesion and metastasis of tumor cells that express high levels of heparan sulfate (2022)

Product Code: 370255-S
Author List: Alexandra De Zutter, Luna Dillemans, Nele Berghmans, Sam Noppen, Helena Crijns, Paulien Verscheure, Janne Verhaegen, Erik Martens, Lotte Vanbrabant, Noëmie Pörtner, Dominique Schols, Paul Proost, Sofie Struyf
Journal: International Journal of Biological Macromolecules

Heparanase is a putative mediator of endothelial glycocalyx damage in COVID-19 – a proof-of-concept study (2022)

Product Code: 370255-S; 370255-1; 370730-S; 370730-1
Author List: Carolin Christina Drost, Alexandros Rovas, Irina Osiaevi, Matthias Rauen, Johan van der Vlag, Baranca Buijsers, Rustem Salmenov, Alexander Lukasz, Hermann Pavenstädt, Wolfgang A. Linke, and Philipp Kümpers
Journal: Frontiers in Immunology, 13

Evaluation of the cytotoxicity of cationic polymers on glioblastoma cancer stem cells. (2022)

Product Code: 370255-S
Author List: Conor McCartin, Juliette Blumberger, Candice Dussouillez, Patricia Fernandez de Larrinoa, Monique Dontenwill, Christel Herold-Mende, Philippe Lavalle, Béatrice Heurtault, Stéphane Bellemin-Laponnaz, Sylvie Fournel and Antoine Kichler
Journal: Journal of Functional Biomaterials, 14(1), 17.

Microphysiological endothelial models to characterize subcutaneous drug absorption (2022)

Product Code: 370255-S
Author List: Giovanni S. Offeddu, Jean Carlos Serrano, Zhengpeng Wan, Mark A. Bryniarski, Sara C. Humphreys, Sophia W. Chen, Hamsini Dhoolypala, Kip Conner and Roger D. Kamm
Journal: ALTEX

Rhamnan Sulfate Reduces Atherosclerotic Plaque Formation and Vascular Inflammation. bioRxiv. (2022)

Product Code: 370255-1, 370255-S
Author List: Patil, N. P., Gomez-Hernandez, A., Zhang, F., Cancel, L., Feng, X., Yan, L., ... & Baker, A. B.
Journal: bioRxiv.

Heparan sulfate dependent binding of plasmatic von Willebrand factor to blood circulating melanoma cells attenuates metastasis (2022)

Product Code: 370255-S
Author List: YuanyuanWangab1XiaoboLiua1TobiasObseraAlexander T.BaueraMartinHeyescSarahStarzonekdMinaZulalaKarenaOpitzaLeonieOtteSabineRiethdorfeTobiasLangedKlausPanteleGerdBendascStefan W.SchneideraMarionKusche-GullbergfChristianGorzelannya
Journal: ELSEVIER SCIENCE DIRECT

Binding of the human antioxidation protein α1-microglobulin (A1M) to heparin and heparan sulfate. Mapping of binding site, molecular and functional characterization, and co-localization in vivo and in vitro (2021)

Product Code: 370255-S; 370255-1
Author List: Bergwik, J., Kristiansson, A., Larsson, J., Ekström, S., Åkerström, B., & Allhorn, M.
Journal: Redox biology, 41

Proteoglycan synthesis in conserved oligomeric Golgi subunit deficient HEK293T cells is affected differently, depending on the lacking subunit. (2021)

Product Code: 370255-S
Author List: Adusmalli, R., Åsheim, H. C., Lupashin, V., Blackburn, J. B., & Prydz, K.

Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire (2021)

Product Code: 370255-S; 370255-1
Author List: Dill, V., Pfaff, F., Zimmer, A., Beer, M., & Eschbaumer, M.
Journal: bioRxiv

Glycosaminoglycans located on neutrophils and monocytes impact on CXCL8-and CCL2-induced cell migration (2021)

Product Code: 370255-S
Author List: Gerlza, T., Nagele, M., Mihalic, Z., Trojacher, C., & Kungl, A.
Journal: Cytokine

Heparan Sulfate Deficiency in Cartilage: Enhanced BMP-Sensitivity, Proteoglycan Production and an Anti-Apoptotic Expression Signature after Loading. (2021)

Product Code: 370255-S
Author List: Gerstner, M., Severmann, A. C., Chasan, S., Vortkamp, A., & Richter, W.
Journal: International journal of molecular sciences

Heparan sulfate is essential for thymus growth. (2021)

Product Code: 370255-S; 370255-1
Author List: Hsu, H. P., Chen, Y. T., Chen, Y. Y., Lin, C. Y., Chen, P. Y., Liao, S. Y., ... & Dzhagalov, I. L
Journal: Journal of Biological Chemistry, 296, 100419.

Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules (2021)

Product Code: 370255-S; 370255-1
Author List: Imura-Kishi, K., Uchida, A., Tsunekawa, N., Suzuki, H., Takase, H. M., Hirate, Y., ... & Kanai, Y.
Journal: Scientific reports

Medullary stromal cells synergize their production and capture of CCL21 for T-cell emigration from neonatal mouse thymus. (2021)

Product Code: 370255-S
Author List: Medullary stromal cells synergize their production and capture of CCL21 for T-cell emigration from neonatal mouse thymus
Journal: Blood Advances

 Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7 (2021)

Product Code: 370255-S
Author List: Kim, M. G., Kim, C. L., Kim, Y. S., Jang, J. W., & Lee, G. M.
Journal: Scientific reports,

The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination. (2021)

Product Code: 370255-S; 370255-1
Author List: Offeddu, G. S., Hajal, C., Foley, C. R., Wan, Z., Ibrahim, L., Coughlin, M. F., & Kamm, R. D.
Journal: Communications biology

Glycocalyx sialic acids regulate Nrf2-mediated signaling by fluid shear stress in human endothelial cells. (2021)

Product Code: 370255-S; 370255-1
Author List: Psefteli, P. M., Kitscha, P., Vizcay, G., Fleck, R., Chapple, S. J., Mann, G. E., ... & Siow, R. C.
Journal: Redox biology

Heparan Sulfate Is a Cellular Receptor for Enteric Human Adenoviruses. (2021)

Product Code: 370255-S; 370255-1
Author List: Rajan, A., Palm, E., Trulsson, F., Mundigl, S., Becker, M., Persson, B. D., ... & Lenman, A.
Journal: Viruses

VISTA is an activating receptor in human monocytes (2021)

Product Code: 370255-S
Author List: Rogers, B. M., Smith, L., Dezso, Z., Shi, X., DiGiammarino, E., Nguyen, D., ... & Starbeck-Miller, G. R.
Journal: Journal of Experimental Medicine, 218(8), e20201601

Secretoglobin 3A2 eliminates human cancer cells through pyroptosis (2021)

Product Code: 370255-S; 370255-1
Author List: Shigetoshi, Y., Shun, N., Xu, L., Pilon, A. L., & Shioko, K.
Journal:  Cell Death Discovery

Endothelial glycocalyx shields the interaction of SARS-CoV-2 spike protein with ACE2 receptors (2021)

Product Code: 370255-S
Author List: Targosz-Korecka, M., Kubisiak, A., Kloska, D., Kopacz, A., Grochot-Przeczek, A., & Szymonski, M.
Journal: Scientific Reports

EXTRACELLULAR ACIDOSIS ENHANCES ZIKA VIRUS INFECTION BOTH IN HUMAN CELLS AND EX VIVO TISSUE CULTURES FROM FEMALE REPRODUCTIVE TRACT. (2021)

Product Code: 370255-S
Author List: Varese, A., Dantas, E., Paletta, A., Fitzgerald, W., Di Diego García, F., Cabrerizo, G., ... & Ceballos, A. (2021).
Journal: Emerging Microbes & Infections,

Secretoglobin 3A2 eliminates human cancer cells through pyroptosis (2021)

Product Code: 370255-S; 370255-1
Author List: Yokoyama, S., Nakayama, S., Xu, L., Pilon, A. L., & Kimura, S.
Journal: Cell death discovery,

The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients (2020)

Product Code: 370255-1, 370255-s
Author List: BenoitYou, FrédéricMercier, EricAssenat, CaroleLanglois-Jacques, OlivierGlehen, JulienSoulé, LéaPayen, VahanKepenekian, MarieDupuy, FannyBelouin, EricMorency, VéroniqueSaywell, MaudFlacelière, PhilippeElies, PierreLiaud, ThibaultMazard, DelphineMaucort-Boulch, WinstonTan, BérengèreVire, LaurentVilleneuve, MarcYchou, ManishKohli, DominiqueJoubert and AlexandrePrieurs
Journal: EBioMedicine

Genome-wide CRISPR-Cas9 screen reveals common factors in dsRNA and Sindbis virus-induced cell death (2020)

Product Code: 370255-S, 370255-1
Author List: Olivier Petitjean, Erika Girardi, Richard Patryk Ngondo, Vladimir Lupashin, Sébastien Pfeffer
Journal: bioRXiv

Reveals the Importance of the Heparan Sulfate Pathway and the Conserved Oligomeric Golgi Complex for Synthetic Double-Stranded RNA Uptake and Sindbis Virus Infection. (2020)

Product Code: 370255-S; 370255-1
Author List: Petitjean, O., Girardi, E., Ngondo, R. P., Lupashin, V., & Pfeffer, S.
Journal: mSphere

Reduced Sulfation Enhanced Oxytosis and Ferroptosis in Mouse Hippocampal HT22 Cells (2020)

Product Code: 370255-1, 370255-S
Author List: Haruna Nagase, Yasuhiro Katagiri, Kentaro Oh-hashi, Herbert M. Geller and Yoko Hirata
Journal: Biomolecules

Chikungunya virus strains from each genetic clade bind sulfated glycosaminoglycans as attachment factors (2020)

Product Code: 370255-S; 370255-1
Author List: McAllister, N., Liu, Y., Silva, L. M., Lentscher, A. J., Chai, W., Wu, N., ... & Dermody, T. S.
Journal: Journal of virology

Heparan sulfate attachment receptor is a major selection factor for attenuated enterovirus 71 mutants during cell culture adaptation (2020)

Product Code: 370255-S, 370255-1
Author List: Kyousuke Kobayashi, Katsumi Mizuta, Satoshi Koike
Journal: Plos Pathogens

Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44 (2020)

Product Code: 370260-1, 370260-S, 370255-1, 370255-S
Author List: Jing-You Guo, Chu-Hsuan Chiu, Mei-Jung Wang, Fu-An Li & Jeou-Yuan Chen
Journal: Journal of Biomedical Science

 Reduced cellular binding affinity has profoundly different impacts on the spread of distinct poxviruses (2020)

Product Code: 370255-S
Author List: Flores, E. B., Bartee, M. Y., & Bartee, E.
Journal: PloS one

Expression and Its Role in Il-4 Driven Multinucleated Giant Cell Formation (2020)

Product Code: 370255-S; 370255-1
Author List: Brooks, P. J.
Journal: Doctoral dissertation

Glycosaminoglycans from Alzheimer’s disease hippocampus have altered capacities to bind and regulate growth factors activities and to bind tau (2019)

Product Code: 370255-S, 370255-1
Author List: Huynh, M. B., Ouidja, M. O., Chantepie, S., Carpentier, G., Maïza, A., Zhang, G., ... & Papy-Garcia, D.
Journal: PloS one, 14(1), e0209573

Dual roles of heparanase in human carotid plaque calcification (2019)

Product Code: 370255-S, 370255-1
Author List: Aldi, S., Eriksson, L., Kronqvist, M., Lengquist, M., Löfling, M., Folkersen, L., ... & Österholm, C.
Journal: Atherosclerosis

Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro (2019)

Product Code: 370255-S, 370255-1
Author List: Ashworth, J. C., Thompson, J. L., James, J. R., Slater, C. E., Pijuan-Galitó, S., Lis-Slimak, K., ... & Tassieri, M.
Journal: Matrix Biology

Heparan sulfate in human cutaneous Meissner's and Pacinian corpuscles (2019)

Product Code: 370730-1, 370730-s, 370255-1, 370255-S
Author List: Jorge García‐Piqueras Olivia García‐Suárez Yolanda García‐Mesa Beatriz García‐Fernandez Luis M. Quirós Ramón Cobo Benjamin Martín‐Biedma Jorge Feito José A. Vega
Journal: The anatomical record. Advances in integrative anatomy and evolutionary biology.

Contribution of syndecans to cellular uptake and fibrillation of α-synuclein and tau (2019)

Product Code: 370255-1, 370255-S
Author List: Hudák, A., Kusz, E., Domonkos, I., Jósvay, K., Kodamullil, A. T., Szilák, L., ... & Letoha, T.
Journal: Scientific reports, 9(1), 1-19

Contribution of syndecans to cellular internalization and fibrillation of amyloid-β (1–42) (2019)

Product Code: 370255-S, 370255-1
Author List: Letoha, T., Hudák, A., Kusz, E., Pettkó-Szandtner, A., Domonkos, I., Jósvay, K., ... & Szilák, L.
Journal: Scientific Reports, 9(1), 1393

Heparan sulfate negatively regulates intestinal stem cell proliferation in Drosophila adult midgut (2019)

Product Code: 370255-S, 370255-1
Author List: Ma, H., Zhao, H., Liu, F., Zhao, H., Kong, R., Shi, L., ... & Li, Z.
Journal:  Biology Open, 8(10), bio047126

Functional Role of Glycosaminoglycans in Decellularized Lung Extracellular Matrix (2019)

Product Code: 370255-1, 370255-S
Author List: Uhl, F. E., Zhang, F., Pouliot, R. A., Uriarte, J. J., Enes, S. R., Han, X., ... & Hallgren, O.
Journal: Acta Biomaterialia

Heparan sulfate maintains adult midgut homeostasis in Drosophila (2019)

Product Code: 370255-1, 370255-s, 370260-S
Author List: Min Wei, Lin Shi, Ruiyan Kong, Hang Zhao and Zhouhua Li
Journal: Cell Biology International

Vascular bioengineering of scaffolds derived from human discarded transplant kidneys using human pluripotent stem cell‐derived endothelium. (2018)

Product Code: 370255-S, 370255-1
Author List: Leuning, D. G., Witjas, F. M., Maanaoui, M., de Graaf, A. M., Lievers, E., Geuens, T., ... & de Boer, H.
Journal: American Journal of Transplantation.

Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation (2018)

Product Code: 370255-S
Author List: Harding, I. C., Mitra, R., Mensah, S. A., Herman, I. M., & Ebong, E. E.
Journal: Journal of translational medicine, 16(1), 364

Glycoprotein K8.1A of Kaposi's sarcoma-associated herpesvirus is a critical B cell tropism determinant, independent of its heparan sulfate binding activity (2018)

Product Code: 370255-S, 370255-1
Author List: Dollery, S. J., Santiago-Crespo, R. J., Chatterjee, D., & Berger, E. A.
Journal: Journal of Virology, JVI-01876

Hemorrhagic shock drives glycocalyx, barrier and organ dysfunction early after polytrauma (2017)

Product Code: 370255-1, 370255-S
Author List: Wiegner, R., Braun, C. K., Denk, S., Mayer, B., Cinelli, P., Radermacher, P., ... & Huber-Lang, M.
Journal:  Journal of Critical Care

The glycocalyx regulates the uptake of nanoparticles by human endothelial cells in vitro. (2017)

Product Code: 370255-1, 370255-S
Author List: Möckl, L., Hirn, S., Torrano, A. A., Uhl, B., Bräuchle, C., & Krombach, F.
Journal: Nanomedicine, (0).

Control of cell shape, neurite outgrowth, and migration by a Nogo-A/HSPG Interaction (2017)

Product Code: 370255-1, 370255-S
Author List: Kempf, A., Boda, E., Kwok, J. C., Fritz, R., Grande, V., Kaelin, A. M., ... & Fawcett, J. W.
Journal: Developmental cell, 43(1), 24-34

The CYCLOCALYX study: Ovulatory cycle affects circulating compartments of the endothelial glycocalyx in blood. (2017)

Product Code: 370255-1, 370255-S
Author List: Hulde, N., Rogenhofer, N., Brettner, F., Eckert, N. C., Götzfried, I., Nguyen, T., ... & Dendorfer, A.
Journal: American Journal of Reproductive Immunology.

Systemic monocyte chemotactic protein-1 inhibition modifies renal macrophages and restores glomerular endothelial glycocalyx and barrier function in diabetic nephropathy (2017)

Product Code: 370255-1, 370255-S
Author List: Boels, M. G., Koudijs, A., Avramut, M. C., Sol, W. M., Wang, G., van Oeveren-Rietdijk, A. M., ... & Eulberg, D.
Journal: The American Journal of Pathology

Modulation of glycosaminoglycans affects PrPSc metabolism but does not block PrPSc uptake. (2015)

Product Code: 370255-1, 370255-S
Author List: Wolf, H., Graßmann, A., Bester, R., Hossinger, A., Möhl, C., Paulsen, L., ... & Vorberg, I.
Journal: Journal of virology, 89(19), 9853-9864.

The CD8-Derived Chemokine XCL1/Lymphotactin Is a Conformation-Dependent, Broad-Spectrum Inhibitor of HIV-1. (2013)

Product Code: 370255-1, 370255-S
Author List: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman BF, et al.
Journal: PLoS Pathog 9(12): e1003852. doi:10.1371/journal.ppat.1003852

Interaction between mouse adenovirus type 1 and cell surface heparan sulfate proteoglycans. (2012)

Product Code: 370255-1, 370255-S
Author List: Lenaerts, L., van Dam, W., Persoons, L., & Naesens, L.
Journal: PloS one, 7(2), e31454.

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