Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NHEJ1-Knockout HeLa cells (KO, Cat# LC831412) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1 monoclonal antibody TA502002 (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
HEK293T cells transfected with either RC203393 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NHEJ1 antibody (TA502002), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NHEJ1 antibody (TA502002), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1. Positive lysates LY403031 (100ug) and LC403031 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.
Anti-NHEJ1 mouse monoclonal antibody (TA502002) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1 (RC203393).
Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody (TA502002), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).