IF image of Hela cell stained with SMAD4 Antibody (C-term) (Cat#TA324984).Hela cells were incubated with SMAD4 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) (Cat. #TA324984). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).
SMAD4 Antibody (C-term) (Cat. #TA324984) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.