HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3GL1 (RC201552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3GL1. Positive lysates LY418946 (100ug) and LC418946 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH3GL1 monoclonal antibody.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SH3GL1-Knockout 293T cells (KO, Cat# LC812166) were separated by SDS-PAGE and immunoblotted with anti-SH3GL1 monoclonal antibody TA501491, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Flow cytometric Analysis of Hela cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SH3GL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501491)
Anti-SH3GL1 mouse monoclonal antibody (TA501491) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3GL1 (RC201552).
Flow cytometric Analysis of Jurkat cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).