HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2. Positive lysates LY415756 (100ug) and LC415756 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503577)
Anti-SEC14L2 mouse monoclonal antibody (TA503577) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2 (RC215994).
HEK293T cells transfected with either RC215994 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody (TA503577), and then analyzed by flow cytometry.