Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
Anti-SAMHD1 mouse monoclonal antibody (TA501953) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).
Figure from citation: Immunofluorescence of SAMHD1 protein level by using anti-SAMHD1 antibody in human CD4+ T cells. View Citation
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SAMHD1 monoclonal antibody (1:200).
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SAMHD1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells transfected with either RC206013 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAMHD1 antibody (TA501953), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA501953), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).