Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and PTPN1-Knockout Hela cells (KO, Cat# LC810193) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody TA503188. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503188)
HEK293T cells transfected with either RC204902 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPN1 antibody (TA503188), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN1 (RC204902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN1. Positive lysates LY419087 (100ug) and LC419087 (20ug) can be purchased separately from OriGene.
Anti-PTPN1 mouse monoclonal antibody (TA503188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1 (RC204902).