Immunohistochemistry (Paraffin-embedded sections) - Protein Phosphatase 1 beta antibody [EP1804Y]; Paraffin embedded human muscle labelled with TA300926 at 1/100 - 1/250 dilution
Western blot - Protein Phosphatase 1 beta antibody [EP1804Y]; Anti-Protein Phosphatase 1 beta antibody [EP1804Y] at 1/200000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
Flow Cytometry-Anti-Protein Phosphatase 1 beta antibody (TA300926); Overlay histogram showing HeLa cells stained with TA300926 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.