Flow cytometric Analysis of Hela cells, using anti-PRKD2 antibody (TA501671), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2. Positive lysates LY402555 (100ug) and LC402555 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC215335 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody (TA501671), and then analyzed by flow cytometry.