Standard curve for ELISA analysis with PON1 recombinant protein (dilution range from 0.8ng/ml to 200ng/ml) using PON1 Capture Antibody (Cat# TA502610) at 5ug/ml and HRP conjugated PON1 Detection mAb (Cat# TA502528/TA502611) at 0.16ug/ml.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (RC210356, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1. Positive lysates LY400156 (100ug) and LC400156 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Jurkat cells, using anti-PON1 antibody (TA502610), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502610)
HEK293T cells transfected with either RC210356 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody (TA502610), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PON1 antibody (TA502610), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).