Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PKMYT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Flow cytometric Analysis of Hela cells, using anti-PKMYT1 antibody (TA500839), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PKMYT1 antibody (TA500839), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-PKMYT1 mouse monoclonal antibody (TA500839) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKMYT1 (RC222657).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKMYT1 (RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1. Positive lysates LY401358 (100ug) and LC401358 (20ug) can be purchased separately from OriGene.