Upper panel, WB of GFP fusion protein expression in Panc-1 cells by using anti-GFP antibody. Lanes 1 and 5: non-transfected cells; lanes 2 and 6: GFP-PKD-transfected cells; lanes 3 and 7: GFP-PKD2-transfected cells; lanes 4 and 8: GFP-PKD3 transfected cells. Center panel, WB of GFP fusion protein expression in Panc-1 cells by using PKD3 N-term (TA324894) and C-term (AP7025b) antibodies. Lower panel, IF of GFP-PKD3 protein expression in Panc-1 cells by using TA324894 and AP7025b antibodies.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-PKCnu Pab (Cat. #TA324894) in lysate of HL60 cells stimulated with PMA (lane A) and mouse brain tissue lysate (lane B). PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.