Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PACSIN3-Knockout 293T cells (KO, Cat# LC812043) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3 monoclonal antibody TA502215, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3. Positive lysates LY414084 (100ug) and LC414084 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC204103 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PACSIN3 antibody (TA502215), and then analyzed by flow cytometry.