IF image of C2C12 cell stained with ENO1 Antibody (Center) (Cat#TA324847).C2C12 cells were incubated with ENO1 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). ENO1 immunoreactivity is localized to Cytoplasm significantly.
ENO1 Antibody (Center) (Cat. #TA324847) western blot analysis in Jurkat, MCF-7, U251, mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).
Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.