HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH2 (RC207170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH2. Positive lysates LY419743 (100ug) and LC419743 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503775)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDH2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503775)
HEK293T cells transfected with either RC207170 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDH2 antibody (TA503775), and then analyzed by flow cytometry.