HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYM (RC218123, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYM. Positive lysates LY419685 (100ug) and LC419685 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501433, Dilution 1:50)
Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-CRYM monoclonal antibody (1:200).
Anti-CRYM mouse monoclonal antibody (TA501433) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYM (RC218123).