HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (RC204290, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB. Positive lysates LY409455 (100ug) and LC409455 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC204290 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody (TA502179), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-MMAB antibody (TA502179), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).